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Forum: Illumina/Solexa 05-14-2014, 11:25 AM
Replies: 4
Views: 4,965
Posted By spf2
I used NEB's NEBNext® Ultra™ DNA Library Prep Kit...

I used NEB's NEBNext® Ultra™ DNA Library Prep Kit for Illumina to generate nucleosome libraries for the yeast. They looked great on the bio analyzer/qubit, but over clustered on the miseq resulting...
Forum: Illumina/Solexa 05-07-2014, 11:11 AM
Replies: 6
Views: 6,613
Posted By spf2
MiSeq Run, Problem with Index reads

I just ran a miseq run using NEBNext Ultra DNA prep, (the bio analyzer results looked fine prior to sequencing).

We sequenced 6 samples ( 3 samples X 2 duplicates) all with different index...
Forum: Illumina/Solexa 09-17-2013, 12:30 PM
Replies: 4
Views: 4,965
Posted By spf2
Best method to prepare 100-200 bp DNA sample (Micrococcal Nuclease Digested)

Hi Everyone,

We're trying to map the nucleosome occupancy of a yeast. At the moment, I have micrococcal nuclease digested DNA fragments. These are around 100-200 bp long and I was wondering what...
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