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Forum: Sample Prep / Library Generation 08-02-2017, 04:23 AM
Replies: 7
Views: 2,102
Posted By GMDickson
Hi jpleonard2000, Did you ever develop a...

Hi jpleonard2000,

Did you ever develop a protocol to do this (using ligation to add the adaptors rather than a PCR)?

Thanks.
Forum: Illumina/Solexa 07-28-2017, 02:30 AM
Replies: 13
Views: 1,380
Posted By GMDickson
:D You should get a badge of thanks!

:D You should get a badge of thanks!
Forum: Illumina/Solexa 07-28-2017, 02:08 AM
Replies: 13
Views: 1,380
Posted By GMDickson
Many, many thanks nucacidhunter. This is...

Many, many thanks nucacidhunter. This is reassuring. I think we will try for approach 3 as we were not expecting qualitative results. They would be great but for now I don't think they will be...
Forum: Illumina/Solexa 07-27-2017, 07:50 AM
Replies: 13
Views: 1,380
Posted By GMDickson
I know :( I am worried about that as well but not...

I know :( I am worried about that as well but not sure how to avoid it. All of the samples will be of the similar but I am trying to work out how much product we are getting out from the first PCR at...
Forum: Illumina/Solexa 07-27-2017, 06:27 AM
Replies: 13
Views: 1,380
Posted By GMDickson
Thanks HESmith. This was the original idea I have...

Thanks HESmith. This was the original idea I have been toying with.

The thought of ligating the adaptors on latterly was really just to keep costs down for the many samples we will need to run. I...
Forum: Illumina/Solexa 07-27-2017, 05:16 AM
Replies: 13
Views: 1,380
Posted By GMDickson
Prep of samples for MiSeq without 2nd PCR query

Hi Nucacidhunter,

Thanks for the info. I've been looking at the forked adaptor to try and increase the proportion of P1/P2 fragments (or P5/P7) but I was wondering if it is possible to just ligate...
Forum: Illumina/Solexa 07-27-2017, 12:17 AM
Replies: 13
Views: 1,380
Posted By GMDickson
Prep of samples for MiSeq without 2nd PCR query

Hi HESmith,

Many thanks for your response.

Yes, we are using PCR to produce the amplicons initially but would prefer to avoid further PCRs usually necessary to attach the sequncing...
Forum: Illumina/Solexa 07-26-2017, 09:19 AM
Replies: 13
Views: 1,380
Posted By GMDickson
Preparation for amplicon sequencing without PCR

Hi all,
I have had a good look through the forum posts and don't seem to find the same questions I have about preparing amplicons for sequencing (on a MiSeq) without using a 2-step PCR.

I need to...
Forum: Bioinformatics 04-21-2014, 08:29 AM
Replies: 1
Views: 1,065
Posted By GMDickson
Data format - Unusable to R and Plink

Hullo! Apologies for the seemingly basic question but I am in knots over this and hope there is some help.

I have recently 'inherited' an Illumina dataset containing about 36,000SNP genotypes for...
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