SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 25 of 84
Search took 0.01 seconds.
Search: Posts Made By: scbaker
Forum: Ion Torrent 10-16-2013, 11:57 AM
Replies: 121
Views: 98,722
Posted By scbaker
I agree with Jeremy that this probably isn't...

I agree with Jeremy that this probably isn't 'vaporware', but it has definitely been delayed (10 months and counting...) In terms of output, Ion Torrent is now officially saying it will launch with...
Forum: General 10-01-2013, 07:26 AM
Replies: 6
Views: 2,903
Posted By scbaker
[Warning: semi-commercial post] The AllSeq...

[Warning: semi-commercial post]

The AllSeq Knowledge Bank (formerly BlueSEQ) is a neutral and fairly comprehensive compilation of the information that you're looking for: platform performance...
Forum: Illumina/Solexa 09-16-2013, 08:32 AM
Replies: 2
Views: 3,742
Posted By scbaker
I agree. If you're outsourcing the project,...

I agree. If you're outsourcing the project, there's absolutely no reason not to pick the best instrument for the job. I'd look for a provider that has a HiSeq.
Forum: Illumina/Solexa 09-08-2013, 04:16 PM
Replies: 4
Views: 14,403
Posted By scbaker
They are somewhat similar, but here are the basic...

They are somewhat similar, but here are the basic differences:

Genome Analyzer - original machine; still being sold, but no longer being updated; probably not the best choice
HiSeq - main...
Forum: Illumina/Solexa 09-08-2013, 01:53 PM
Replies: 4
Views: 14,403
Posted By scbaker
They all use basically the same chemistry...

They all use basically the same chemistry (sequencing-by-synthesis), but there are many differences in terms of the mechanics and optics of the various instruments. More importantly for the end user...
Forum: Service Providers 08-26-2013, 10:17 AM
Replies: 3
Views: 2,060
Posted By scbaker
I guess I wouldn't call that a new company, just...

I guess I wouldn't call that a new company, just an obvious extension of the service business they already run. They've been doing anything they can to hurt CG for a long time now, and Moleculo is...
Forum: Service Providers 08-26-2013, 10:05 AM
Replies: 3
Views: 2,060
Posted By scbaker
I've not heard any rumors of Illumina setting up...

I've not heard any rumors of Illumina setting up a new company, but they've publicly stated that they will be expanding their sequencing services capacity by reopening the services group that used to...
Forum: General 07-16-2013, 02:33 PM
Replies: 20
Views: 6,362
Posted By scbaker
While I'm expecting and eagerly awaiting...

While I'm expecting and eagerly awaiting continued price drops, Neil has a point about the 'laziness' of some (most?) NGS experiments. There have been some pretty weak experimental designs where the...
Forum: General 07-16-2013, 02:28 PM
Replies: 20
Views: 6,362
Posted By scbaker
Agreed. The price drop WILL stop, but I think it...

Agreed. The price drop WILL stop, but I think it will make a huge difference whether it stops at $1000, $100 or $10 for a whole human genome. Same goes for the ag market you mentioned - lots of...
Forum: General 07-16-2013, 01:15 PM
Replies: 20
Views: 6,362
Posted By scbaker
The lack of dropping costs is purely due to...

The lack of dropping costs is purely due to market forces. I explain it in more detail at my blog: http://biotechmarketer.blogspot.com/2013/05/neil-hall-is-responsible-for-rising-ngs.html

tl;dr...
Forum: Illumina/Solexa 07-11-2013, 11:31 AM
Replies: 2
Views: 1,472
Posted By scbaker
First, I'm not sure the MiSeq has really hit the...

First, I'm not sure the MiSeq has really hit the 25M read mark yet. Their spec is for 15M reads, and that fits with getting ~8Gb with 2X250 reads. Having said that...

There's certainly nothing...
Forum: RNA Sequencing 07-03-2013, 10:12 AM
Replies: 4
Views: 1,882
Posted By scbaker
Well, running your samples in two separate runs...

Well, running your samples in two separate runs will add SOME variability, so I would avoid it if possible. I would recommend doing one of the following:
1) 4 lanes on a single 2000 flow cell
2) a...
Forum: RNA Sequencing 07-03-2013, 08:08 AM
Replies: 4
Views: 1,882
Posted By scbaker
Running your samples on two separate machines...

Running your samples on two separate machines will add a bit of technical variability, but since all of your samples are multiplexed together, my guess is that it won't have a significant impact on...
Forum: General 05-29-2013, 12:45 PM
Replies: 7
Views: 1,855
Posted By scbaker
Yeah, that definitely meshes with what I've seen....

Yeah, that definitely meshes with what I've seen. But given that, it's pretty remarkable that HiSeq's are running ~60% of full capacity (where 100% probably isn't even achievable as all machines have...
Forum: General 05-24-2013, 12:33 PM
Replies: 7
Views: 1,855
Posted By scbaker
Thanks for the mention, kopi-o! This is a...

Thanks for the mention, kopi-o!

This is a really interesting question. We’re working on tools that will allow us to estimate things like this in the future, but for now we can make a ‘back of the...
Forum: Pacific Biosciences 05-24-2013, 10:40 AM
Replies: 11
Views: 3,122
Posted By scbaker
PacBio lists all of their providers here:...

PacBio lists all of their providers here: http://www.pacificbiosciences.com/support/sequencing_provider/

It looks like DNA Link in Korea might be an option for you.
Forum: Illumina/Solexa 05-07-2013, 09:21 PM
Replies: 12
Views: 24,772
Posted By scbaker
I left Illumina a little over two years ago, so I...

I left Illumina a little over two years ago, so I can't answer specifically about their kits, but I've seen a number of solutions that use rRNA depletion (or avoidance). I haven't used any of these...
Forum: Pacific Biosciences 03-26-2013, 09:48 AM
Replies: 7
Views: 5,717
Posted By scbaker
Ah, that makes perfect sense - thanks!

Ah, that makes perfect sense - thanks!
Forum: Pacific Biosciences 03-25-2013, 08:09 AM
Replies: 7
Views: 5,717
Posted By scbaker
I think it's due to both the 'single molecule'...

I think it's due to both the 'single molecule' and 'real time' nature of the system. The RS is essentially taking a video of the polymerase adding nucleotides in real time. As this happens very...
Forum: Pacific Biosciences 03-17-2013, 09:35 AM
Replies: 6
Views: 4,147
Posted By scbaker
After a nice conversation with PacBio CSO Jonas...

After a nice conversation with PacBio CSO Jonas Korlach, I've updated the specs we maintain at BlueSEQ: http://blueseq.com/knowledgebank/sequencing-platforms/pacific-biosciences/

Runs can be...
Forum: Events / Conferences 03-03-2013, 12:13 PM
Replies: 1
Views: 875
Posted By scbaker
[Quasi Commercial Post Warning] Thanks for...

[Quasi Commercial Post Warning]

Thanks for letting us know about this meeting. We've added it to our list of NGS Conferences (over 50 and counting for 2013):...
Forum: The Pipeline 02-22-2013, 04:28 PM
Replies: 19
Views: 18,742
Posted By scbaker
Very interesting. Do you know if they've...

Very interesting. Do you know if they've purchased the IP from $LIFE or if they have any commercialization plans?
Forum: Illumina/Solexa 01-18-2013, 02:29 PM
Replies: 31
Views: 14,168
Posted By scbaker
I'm assuming dilution is involved, similar to...

I'm assuming dilution is involved, similar to Complete Genomics' LFR technology, but perhaps with microfluidics.
Forum: Illumina/Solexa 01-18-2013, 09:40 AM
Replies: 31
Views: 14,168
Posted By scbaker
I think what Moleculo is doing is creating a...

I think what Moleculo is doing is creating a bunch of mini-libraries from long (10-15kb) sequences. Sequences from each mini-library are connected via a unique sequence tag so that the original long...
Forum: Ion Torrent 01-18-2013, 09:36 AM
Replies: 70
Views: 17,784
Posted By scbaker
It's pure speculation on my part, but this could...

It's pure speculation on my part, but this could be the beginning of Roche getting their hands on Ion Torrent. The 'strategic review' could result in a breakup of the company, separating the...
Showing results 1 to 25 of 84

 


All times are GMT -8. The time now is 07:44 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO