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Forum: 454 Pyrosequencing 09-16-2011, 05:21 AM
Replies: 18
Views: 7,728
Posted By uhany
Hi Anthony, Yes capture beads in Lib A...

Hi Anthony,

Yes capture beads in Lib A protocol is different and that is ok. I determine by enriched beads/total no. of beads and usually get good results from up to 30% recovered emulsions....
Forum: 454 Pyrosequencing 08-11-2011, 02:45 AM
Replies: 1
Views: 1,728
Posted By uhany
lisa, Expired DNA capture beads worked fine...

lisa,

Expired DNA capture beads worked fine with me. There is a modified protocol (pcr volume and cycle) in my454 for amplicons <200bp, I think you should try that but that probably needs using...
Forum: 454 Pyrosequencing 06-15-2011, 05:04 AM
Replies: 24
Views: 5,467
Posted By uhany
Hi Aniki White beads that actually covers...

Hi Aniki

White beads that actually covers the brown beads are no worth. They need to be washed off anyway. If you invert the MPC couple of times very slowly they will get dissolved in the...
Forum: 454 Pyrosequencing 08-09-2010, 12:13 AM
Replies: 21
Views: 11,734
Posted By uhany
thanks a lot tplsmith..I was thinking the same as...

thanks a lot tplsmith..I was thinking the same as well..
Forum: 454 Pyrosequencing 08-06-2010, 01:08 AM
Replies: 21
Views: 11,734
Posted By uhany
thanks soulbee

well enrichment primers are from the kits and they are not outdated ..so far I have done everything according to the protocols..so I don't know what went wrong..I tried different kits as...
Forum: 454 Pyrosequencing 08-05-2010, 12:23 AM
Replies: 21
Views: 11,734
Posted By uhany
Hi I am having serious problem in beads...

Hi

I am having serious problem in beads recovery after emulsion PCR. It seems all the beads are getting washed off ....and I have left with no beads for sequencing..does anyone have similar...
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