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Forum: Sample Prep / Library Generation 01-05-2018, 08:37 AM
Replies: 5
Views: 1,277
Posted By lbcbci
Single cell RNA-seq trouble

That would be due to significant lower RNA quantities from T cells than Jurkat cells
Forum: Service Providers 07-08-2014, 12:36 PM
Replies: 162
Views: 143,156
Posted By lbcbci
Great resources! Is it possible to share an...

Great resources!

Is it possible to share an updated list's link by now for these all service providers?

Thanks!
Forum: Sample Prep / Library Generation 03-25-2014, 12:26 PM
Replies: 9
Views: 6,680
Posted By lbcbci
Do you mean they are using HMW for Nextera kit...

Do you mean they are using HMW for Nextera kit and LMW for XT kit?
Thanks,

LL
Forum: Sample Prep / Library Generation 02-21-2014, 08:55 AM
Replies: 14
Views: 11,035
Posted By lbcbci
Thanks a lot, Simone 78. It is highly possible...

Thanks a lot, Simone 78.
It is highly possible illumina's typo problem since 13,000g would be very close to 11,000rpm which is typical number for the community.
But I just curious dose anybody have...
Forum: Sample Prep / Library Generation 02-20-2014, 04:05 PM
Replies: 14
Views: 11,035
Posted By lbcbci
Nextera DNA sample kit protocol

I am recently using Nextera DNA sample kit for library prep. According user guide, the spin speed was set as 1300xg for PCR clean up with Zymo column. Actually there is only about 4000rpm (Eppendorff...
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