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Forum: Illumina/Solexa 09-30-2016, 12:05 AM
Replies: 4
Views: 5,549
Posted By anujgupta
QXT with FFPE

can you share your experimental experiences with QXT for FFPE? Did you modified anything? pls share it to anujksj@gmail.com
Forum: 454 Pyrosequencing 02-15-2011, 08:20 AM
Replies: 6
Views: 3,982
Posted By anujgupta
All depend upon your hands-on expertise. Emulsion...

All depend upon your hands-on expertise. Emulsion breaking specially in LV results in aerosol formation that's why this area is termed as dirty. It should be separated from that used for emulsion...
Forum: 454 Pyrosequencing 02-15-2011, 08:12 AM
Replies: 1
Views: 1,965
Posted By anujgupta
Dear winmicro40, 1. Primer on each bead is...

Dear winmicro40,

1. Primer on each bead is same. Usually it is anti-B adaptor so that B adaptor of the library can bind to it. In case of Lib-A kit of emPCR used for bi-directional amplicon...
Forum: 454 Pyrosequencing 02-10-2011, 01:41 AM
Replies: 9
Views: 2,822
Posted By anujgupta
Agree with Soulbee. I wish to add one more thing...

Agree with Soulbee. I wish to add one more thing that while loading PTP at the time of sequencing run, Do not try to suck and push by pipetting in order to remove bubbles. Pipetting should be in one...
Forum: 454 Pyrosequencing 02-10-2011, 01:29 AM
Replies: 7
Views: 3,185
Posted By anujgupta
They are not binding back. I mentioned.. they are...

They are not binding back. I mentioned.. they are trapped beads, you can separate them out by mild pipetting. Transferring to further tubes as you did also works. I give you one good idea. After...
Forum: 454 Pyrosequencing 02-10-2011, 01:27 AM
Replies: 7
Views: 3,185
Posted By anujgupta
They are not binding back. I mentioned.. they are...

They are not binding back. I mentioned.. they are trapped beads, you can separate them out by mild pipetting. Transferring to further tubes as you did also works. I give you one good idea. After...
Forum: 454 Pyrosequencing 02-02-2011, 07:25 PM
Replies: 7
Views: 3,185
Posted By anujgupta
These white beads are generally good beads, which...

These white beads are generally good beads, which you require. This is not NaOH precipitate. These are trapped beads, which you should remove from brown beads by gently pipetting up and down keeping...
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