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Forum: Illumina/Solexa 01-27-2011, 02:30 PM
Replies: 8
Views: 3,670
Posted By jowenMIT
I agree with Jmorlan about properly annealing the...

I agree with Jmorlan about properly annealing the adapters. I forgot to do this with some homemade adapters, and it resulted in an almost complete lack of proper ligation.
Forum: Illumina/Solexa 01-27-2011, 02:21 PM
Replies: 0
Views: 2,266
Posted By jowenMIT
mRNA fragmentation for SPRIworks

We are making ds-cDNA for automated mRNA-seq library preparation using the SPRIworks robot. After the poly-A selection, our protocol calls for fragmentation at 80 degrees for five minutes using...
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