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Search: Posts Made By: lvcosme
Forum: Bioinformatics 06-06-2012, 06:38 AM
Replies: 30
Views: 8,543
Posted By lvcosme
Nice, lots of answers. We have two computer for...

Nice, lots of answers. We have two computer for analyzes in our lab, but they are suitable to run gene expression analysis (Tophat, cufflinks etc), not de novo assembly, like the one I have to do. ...
Forum: Bioinformatics 06-05-2012, 12:45 PM
Replies: 30
Views: 8,543
Posted By lvcosme
Is anyone using a SSD as swap for de novo...

Is anyone using a SSD as swap for de novo assembly? I have an I7 overclocked to 4.2Ghz, 32GB RAM DDR3 1600, and I also have 3 SSD of 120GB. I was thinking to use one of them as swap, and then I would...
Forum: General 09-20-2011, 01:30 PM
Replies: 0
Views: 1,473
Posted By lvcosme
Forum: Bioinformatics 07-27-2011, 03:25 PM
Replies: 10
Views: 6,521
Posted By lvcosme
oh permissions

I am trying to set it up, but I keep getting this error message:
ln: creating symbolic link `bowtie': Permission denied

I changed the permission of bowtie folders and files but keep getting same...
Forum: Illumina/Solexa 03-07-2011, 09:26 AM
Replies: 2
Views: 2,217
Posted By lvcosme
Thanks. I forgot to mention this is the...

Thanks. I forgot to mention this is the fragmented poly(A+) RNA, not the library. I finish this library, but the final product was too small, so I am doing it again. I did not find any fragmented...
Forum: Illumina/Solexa 03-07-2011, 08:22 AM
Replies: 2
Views: 2,217
Posted By lvcosme
Bioanalyzer electropherogram ~200bp

Hi Everyone,
I am preparing some libraries from 150ng of mRNA. I am using a buffer to fragment my mRNA after isolation. I tried different concentrations of the buffer (from 0.2 to 1uL, also...
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