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Search: Posts Made By: wraithnot
Forum: Illumina/Solexa 02-13-2015, 03:39 PM
Replies: 14
Views: 8,962
Posted By wraithnot
They can favor the amplification of the...

They can favor the amplification of the heterodimers carrying N7xx/N5xx oligos with suppression PCR: N7xx/N5xx http://www.nature.com/app_notes/nmeth/2009/091011/full/nmeth.f.272.html

The...
Forum: Ion Torrent 04-23-2012, 12:03 PM
Replies: 121
Views: 96,451
Posted By wraithnot
A paper that came out yesterday in Nature...

A paper that came out yesterday in Nature Biotechnology may provide some of the answers you are seeking: http://www.nature.com/nbt/journal/vaop/ncurrent/pdf/nbt.2198.pdf
Forum: General 05-18-2011, 12:13 PM
Replies: 14
Views: 3,760
Posted By wraithnot
As a fellow scientist and tinkerer, I completely...

As a fellow scientist and tinkerer, I completely agree with you. However, I once heard a biotech CEO address a similar point from a completely different angle. He basically said that if they gave...
Forum: General 05-18-2011, 11:43 AM
Replies: 39
Views: 21,372
Posted By wraithnot
We're also trying to decide between these two...

We're also trying to decide between these two instruments. I think most of the information you're after can be found here:...
Forum: General 03-16-2011, 12:58 PM
Replies: 7
Views: 1,908
Posted By wraithnot
After a bit more digging, the original premise of...

After a bit more digging, the original premise of this thread could be correct (at least if you limit yourselves to consumable costs). Here's the first paragraph of a press release from Complete...
Forum: General 03-16-2011, 12:24 PM
Replies: 7
Views: 1,908
Posted By wraithnot
I think there is a slight math error in your...

I think there is a slight math error in your calculations. Unless I'm completely misunderstanding something, 1 Tb is about 333 1X 3-Gb human genomes or 11.1 30x human genomes.

The HiSeq 2000...
Forum: Ion Torrent 03-11-2011, 04:07 PM
Replies: 14
Views: 11,295
Posted By wraithnot
Great to hear that the machine works as...

Great to hear that the machine works as advertised! What sort of read lengths did you get? Did the extra 5 Mb come from more sequence reads than expected, longer sequence reads than expected, or...
Forum: Illumina/Solexa 10-25-2010, 06:34 PM
Replies: 4
Views: 1,524
Posted By wraithnot
@kmcarr- thanks for the detailed response. I...

@kmcarr- thanks for the detailed response. I took a closer look at the raw fastq data they gave me. For the sample with two separate insertions spaced 36 bp apart, the second insertion had a clear...
Forum: Illumina/Solexa 10-25-2010, 09:52 AM
Replies: 4
Views: 1,524
Posted By wraithnot
Duplicated bases in 100 bp GA2 reads

Hi All,

I recently found an odd artifact in some 100 bp illumina GA2 reads we got from our sequencing provider. After some initial consternation, I realized that all the raw data contained...
Forum: The Pipeline 08-23-2010, 09:55 AM
Replies: 15
Views: 13,191
Posted By wraithnot
I hate to disagree, but I believe that quote is...

I hate to disagree, but I believe that quote is from a stock analyst named Jon Groberg : "In a research note analyzing the acquisition, Jon Groberg of Macquarie Research said that even though Ion...
Forum: Illumina/Solexa 04-08-2009, 12:32 PM
Replies: 24
Views: 25,166
Posted By wraithnot
Our experiments use 96 separate barcodes. If you...

Our experiments use 96 separate barcodes. If you aren't careful about normalizing the concentrations of each sample when you combine them you can get wildly different amounts of each barcoded...
Forum: Illumina/Solexa 04-07-2009, 11:43 AM
Replies: 24
Views: 25,166
Posted By wraithnot
Multiplexing strategy

This is right up my alley so this seems like a good opportunity to make my first post on this board.

An elegant way to design error correcting bar codes can be found in Hamady et al. Nature...
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