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Forum: RNA Sequencing 03-19-2020, 05:42 AM
Replies: 7
Views: 1,267
Posted By mvheetve
So I used fastp to remove low quality reads,...

So I used fastp to remove low quality reads, adapters, reads that were too short, G-tails, etc... Then remapped using BWA, but this time against the fasta provided by GenoMax.

For the data of the...
Forum: RNA Sequencing 03-16-2020, 07:01 AM
Replies: 7
Views: 1,267
Posted By mvheetve
Thanks, that's a welcome reference. ...

Thanks,

that's a welcome reference.

Regarding the mapping: well I would think so yes. I used a fasta with all known human rRNA sequences (genome and mitochondrial) as reference in BWA. Come to...
Forum: RNA Sequencing 03-16-2020, 06:43 AM
Replies: 7
Views: 1,267
Posted By mvheetve
RE: prep and depletion

Hi Olaf,

thanks for showing an interest :cool:. I did specify the RNA lib prep, they used Illumina RNA Access...
Forum: RNA Sequencing 03-12-2020, 03:40 AM
Replies: 7
Views: 1,267
Posted By mvheetve
Unhappy inexplicable rRNA content

Hi guys,

long time reader, first time poster here. So here's my problem:

Did two RNAseq runs (NextSeq500, RNA Access Illumina prep, 20 samples per run).

The first run had 20 samples, high...
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