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Forum: Epigenetics 03-07-2015, 09:33 PM
Replies: 0
Views: 1,461
Posted By RNAarecrazy
MEDIP-seq: low unique reads

Hi everyone, I mapped my medip-seq on mouse liver genome single end data with Bowtie 2 and only 10% of reads map to the mouse genome once, so only 1 million reads are "unique". Large proportion of...
Forum: Epigenetics 03-07-2015, 09:25 PM
Replies: 3
Views: 2,127
Posted By RNAarecrazy
yes, we routinely sonicate to size 150-200bp for...

yes, we routinely sonicate to size 150-200bp for chip-seq.
Forum: Epigenetics 09-03-2014, 07:55 AM
Replies: 1
Views: 5,659
Posted By RNAarecrazy
Reply from NEB re: ssDNA library prep

I thought this might be helpful for some of you.

Thank you for contacting NEB Technical Support.

Dear Chris,

Your question and answer are...
Forum: Epigenetics 09-03-2014, 07:52 AM
Replies: 1
Views: 2,143
Posted By RNAarecrazy
ssDNA library prep for meDIP

Hi everyone, I am working on meDIP-seq using antibody that recognizes methylated cytosines to pull down methylated regions in the genome.

I did it a bit differently from conventional protocols,...
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