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Forum: Sample Prep / Library Generation 12-01-2015, 03:23 PM
Replies: 23
Views: 7,709
Posted By Angelmass
http://i.imgur.com/oG0990C.jpg Here is a...

http://i.imgur.com/oG0990C.jpg

Here is a helpful diagram that I use when determining bead cleanup concentrations.
Forum: Core Facilities 10-13-2015, 02:04 PM
Replies: 4
Views: 7,574
Posted By Angelmass
We use Biomek FXs and Hamilton Star and Starlets,...

We use Biomek FXs and Hamilton Star and Starlets, but I don't know if that would really solve your cost problem, since they're both pretty large and expensive-looking. Another consideration you might...
Forum: Sanger/Dye Terminator 10-13-2015, 01:55 PM
Replies: 1
Views: 5,353
Posted By Angelmass
Is it possible that your capillaries might be...

Is it possible that your capillaries might be blocked? You say that the Big Dye samples have run fine, but were they in the same wells?

Also, have you looked at the array viewer for the wells of...
Forum: Sanger/Dye Terminator 10-13-2015, 01:50 PM
Replies: 1
Views: 5,045
Posted By Angelmass
Is this the priming region or the amplicon? ...

Is this the priming region or the amplicon?

Hairpins cause secondary structure loops cause DNA polymerase slippage, which can cause frameshift mutations in your sequence when undergoing PCR,...
Forum: Sample Prep / Library Generation 10-13-2015, 12:12 PM
Replies: 5
Views: 3,814
Posted By Angelmass
At the moment, we're sticking with QIAGEN kits...

At the moment, we're sticking with QIAGEN kits simply because the contract that we have stipulates that we use the contractor's protocol for guaranteed results during clinical workflow.

As for...
Forum: Illumina/Solexa 08-11-2015, 08:32 AM
Replies: 6
Views: 1,387
Posted By Angelmass
Ah ok, I guess I'm just out of the loop then.

Ah ok, I guess I'm just out of the loop then.
Forum: Illumina/Solexa 08-11-2015, 08:16 AM
Replies: 6
Views: 1,387
Posted By Angelmass
Both our standing order and a new order are...

Both our standing order and a new order are delayed until "8/17", they said they are switching their internal database system used for order management, and the 17th is an estimate, like yours. Very...
Forum: Sample Prep / Library Generation 07-20-2015, 02:19 PM
Replies: 5
Views: 3,814
Posted By Angelmass
How much outside this range? We are trying to...

How much outside this range? We are trying to isolate 10ml plasma at a time.
Forum: Sample Prep / Library Generation 07-17-2015, 01:36 PM
Replies: 5
Views: 3,814
Posted By Angelmass
ctDNA isolation from plasma

Hey guys,

We're currently looking for alternative ctDNA extraction kits. We currently use QIAGENs QIAamp circulating nucleic acid kit for our plasma extractions (10ml plasma). It does currently...
Forum: Sample Prep / Library Generation 07-16-2015, 10:58 AM
Replies: 3
Views: 1,561
Posted By Angelmass
It is still unclear what types of preps you are...

It is still unclear what types of preps you are doing, but I will say I'm a bit of a newbie in terms of background knowledge, but average about 6 MiSeq runs/week. The only other equipment that we use...
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