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Forum: 454 Pyrosequencing 07-02-2010, 06:43 PM
Replies: 0
Views: 1,329
Posted By entropy
Overcalled A's in my 454 data

I've assembled 780,000 reads of 454 sequence with the Roche gsAssembler program and I've noticed that there are a huge number of overcalled A's. Most of them seem to be associated with runs of A's,...
Forum: General 01-21-2010, 09:18 AM
Replies: 2
Views: 2,941
Posted By entropy
Thanks katy. I'm leaning towards doing it on a...

Thanks katy. I'm leaning towards doing it on a 454 machine because its a de novo sequence we're looking to do.
Forum: General 01-20-2010, 12:39 PM
Replies: 3
Views: 2,193
Posted By entropy
Ok. Good to know. Thanks!

Ok. Good to know. Thanks!
Forum: General 01-17-2010, 08:09 PM
Replies: 2
Views: 2,941
Posted By entropy
Opinion on facilities in Canada?

From what I can tell there are only three places in Canada that do "next generation" sequencing. McGill University, Sick Kids in Toronto, and Michael Smith in BC.

Does anyone have any opinions...
Forum: General 01-17-2010, 08:04 PM
Replies: 3
Views: 2,193
Posted By entropy
Computer hardare recommendations for assembly and annotation?

Hi all.

Just wondering what would be a reasonable workstation for assembling a 5.5bp bacterial genome? I'm leaning towards 454 rather than Illumina or SOLiD, but I assume the later two would...
Forum: Introductions 01-17-2010, 07:19 PM
Replies: 1
Views: 2,398
Posted By entropy
Newbie. May ask a lot of stupid questions.

Hi all.

Well, I've been tasked with sequencing and annotating a bacteria for a postdoc project.

I'm a hair away from defending my PhD in Molecular Genetics so I've got the wet lab skills....
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