Forum: Bioinformatics
01-21-2020, 05:33 PM
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Replies: 1
Views: 838
No. Those p-values usually apply to the...
No. Those p-values usually apply to the confidence in the value of the distinguishing statistic (a statistic which, as it happens, is frequently absent from abstracts and the non-supplementary paper...
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Forum: Bioinformatics
01-21-2020, 05:25 PM
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Replies: 2
Views: 3,468
Have a look at the bcftools...
Have a look at the bcftools (https://samtools.github.io/bcftools/bcftools.html) package. It supports uncompressed and gzip-compressed VCF file formats as input, as well as the BCF format, and...
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Forum: Ion Torrent
08-11-2019, 02:01 AM
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Replies: 19
Views: 9,611
Hobby Money
Customers don't pay return shipping to the UK. That cost is met by ONT, even for flow cells that are being returned from New Zealand. I suppose if you want to be cynical, you could say the return...
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Forum: Bioinformatics
08-08-2019, 04:58 PM
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Replies: 10
Views: 1,902
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Forum: Ion Torrent
08-08-2019, 04:50 PM
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Replies: 19
Views: 9,611
The MinION flow cell return to ONT is so that...
The MinION flow cell return to ONT is so that they can recycle them for use in training and internal research. If you contaminate the flow cells such that they are not safe for posting back, you...
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Forum: Oxford Nanopore
05-10-2019, 02:57 AM
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Replies: 6
Views: 7,266
External access
On the other side of things, a MinION sequencing run won't work unless the computer has access to the outside via non-standard ports, which are used to fetch firmware, and send telemetry data a short...
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Forum: Illumina/Solexa
04-07-2019, 04:34 PM
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Replies: 3
Views: 1,030
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Forum: Oxford Nanopore
04-07-2019, 04:26 PM
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Replies: 2
Views: 4,928
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Forum: Oxford Nanopore
04-07-2019, 04:18 PM
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Replies: 1
Views: 3,566
Just buy new flow cells. Anything from 2017 is...
Just buy new flow cells. Anything from 2017 is likely to be dead, and you'll get more sequence in your first hour with new flow cells than you would have got in 48 hours from working flow cells in...
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Forum: Oxford Nanopore
04-07-2019, 04:12 PM
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Replies: 1
Views: 4,990
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Forum: Oxford Nanopore
04-07-2019, 04:07 PM
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Replies: 1
Views: 3,930
As far as I'm aware, it doesn't matter....
As far as I'm aware, it doesn't matter. Nanopolish uses the called fastq files together with the signal in the fast5 files, so both should work. I expect the main difference between the two fast5...
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Forum: Oxford Nanopore
04-07-2019, 03:55 PM
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Replies: 6
Views: 7,266
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Forum: Oxford Nanopore
04-07-2019, 03:48 PM
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Replies: 2
Views: 4,724
Qubit or Quantus (or other similar...
Qubit or Quantus (or other similar fluorescence-based methods) are pretty much essential for nanopore sequencing. The nanodrop has issues with RNA contamination, and gives concentrations that can be...
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Forum: Oxford Nanopore
04-07-2019, 03:43 PM
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Replies: 2
Views: 3,983
The Starter Pack is not sufficient for doing...
The Starter Pack is not sufficient for doing multiple samples. In ONT's infinite wisdom, they decided to put the rapid kit as an option, rather than the rapid barcoding kit (despite them having...
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Forum: General
02-11-2019, 10:59 PM
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Replies: 3
Views: 3,066
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Forum: Oxford Nanopore
10-19-2018, 04:43 PM
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Replies: 0
Views: 4,870
Flongle flow cells - now shipping
Oxford Nanopore Technologies have started shipping Flongle adapters and flow cells to customers. These are disposable flow cells using fewer pores and a cheaper design ($5000 USD for 48 flow cells)....
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Forum: Oxford Nanopore
09-28-2018, 04:42 AM
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Replies: 17
Views: 10,638
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Forum: Sample Prep / Library Generation
09-04-2018, 04:21 AM
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Replies: 4
Views: 1,970
OpenTrons
If you want cheap, there's the OpenTrons OT-2 (http://opentrons.com/ot-2). The basic system is $3500 USD, with additional modules ordered separately:
single channel pipette $500
8-channel...
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Forum: Bioinformatics
09-04-2018, 03:43 AM
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Replies: 2
Views: 1,158
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Forum: Bioinformatics
08-05-2018, 06:53 AM
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Replies: 1
Views: 1,200
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Forum: Bioinformatics
08-05-2018, 06:32 AM
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Replies: 104
Views: 40,765
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Forum: Oxford Nanopore
05-30-2018, 06:29 AM
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Replies: 1
Views: 4,840
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Forum: Bioinformatics
05-28-2018, 06:20 AM
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Replies: 1
Views: 982
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Forum: Bioinformatics
04-22-2018, 05:31 AM
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Replies: 2
Views: 746
If you don't care about accuracy, just...
If you don't care about accuracy, just concatenate all the fasta sequences into a single large sequence....
More seriously, you're probably not going to get any better continuity from your data...
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Forum: Bioinformatics
04-22-2018, 05:25 AM
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Replies: 13
Views: 2,421
That's not the only reason. Sigma also has...
That's not the only reason. Sigma also has region-specific pricing. Without logging in, I choose the location when I first visit the site (presumably stored as a cookie for subsequent visits), and...
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