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Forum: Illumina/Solexa 02-15-2018, 10:08 AM
Replies: 11
Views: 1,642
Posted By smurugesan
Dear Phillip, Sorry I was not clear in my...

Dear Phillip, Sorry I was not clear in my message. The error was during the demultiplex execution according to the analysis error log. When MSR executes the secondary analysis, the error message was...
Forum: Illumina/Solexa 02-14-2018, 01:12 PM
Replies: 11
Views: 1,642
Posted By smurugesan
Customprimers-SampleSheet error during secondary analysis

Hi everyone,

Yesterday i did a 16s MiSeq run using custom primers, when i tried to demultiplex using samplesheet generated through IEM, i found error during secondary analysis to generate Fastq...
Forum: Illumina/Solexa 08-21-2017, 04:02 AM
Replies: 9
Views: 1,554
Posted By smurugesan
Thank you so much @nucacidhunter. That clears my...

Thank you so much @nucacidhunter. That clears my doubt, thanks again.
Forum: Illumina/Solexa 08-14-2017, 10:06 PM
Replies: 9
Views: 1,554
Posted By smurugesan
Thanks for your explanataion Brian. Its really...

Thanks for your explanataion Brian. Its really helpful. For e.g I have 96 different barcodes in my forward primers, But reverse primer is without barcode, My question is if illumina sequence in both...
Forum: Illumina/Solexa 08-12-2017, 09:06 PM
Replies: 9
Views: 1,554
Posted By smurugesan
Thank you Jessica and Brian for your valuable...

Thank you Jessica and Brian for your valuable suggestions. In Illumina guide, they suggested to sequence an amplicon of 550bp or smaller. So I thought it would be ideal, and actually We are planning...
Forum: Illumina/Solexa 08-09-2017, 03:16 AM
Replies: 9
Views: 1,554
Posted By smurugesan
Great, Thank you. A list of customized forward...

Great, Thank you. A list of customized forward primers with barcodes and Reverse primer without barcode, is it possible to sequence amplicon size of 530bp using 600 v3 kit?
Forum: Illumina/Solexa 08-09-2017, 01:11 AM
Replies: 9
Views: 1,554
Posted By smurugesan
How many number of samples per lane

Hi everyone,

I am a new user to Illumina MI-SEQ, in a single lane how many 16S Amplicons we can mix or in other way, to sequence 1000 samples, how may times we have sequence. Can any one suggest...
Forum: Introductions 08-09-2017, 12:24 AM
Replies: 0
Views: 655
Posted By smurugesan
Hi hello

Hi everyone,

This is selva. I am going to use Illumina Miseq for 16S. Thank you somuch for this forum, it is always helpful to newbies. Keep going.
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