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Forum: Ion Torrent 12-07-2016, 06:44 AM
Replies: 9
Views: 4,530
Posted By lucorum
Thanks! I'm not sure how exactly we update OT2...

Thanks!
I'm not sure how exactly we update OT2 soft. However, it has Hi-Q kit in its menu, so its software can't be really old, and an update was performed at least once. I should ask my colleagues...
Forum: Ion Torrent 12-02-2016, 05:34 AM
Replies: 9
Views: 4,530
Posted By lucorum
r.rosati, about OT2: I tried to download the...

r.rosati, about OT2:
I tried to download the logs right after the run, so the instrument was continuosly turned on and shouldn't lose data. I formatted two different flash drives in FAT32 (FAT32 is...
Forum: Ion Torrent 12-01-2016, 06:02 AM
Replies: 9
Views: 4,530
Posted By lucorum
Thank you! I'm sorry, I didn't...

Thank you!



I'm sorry, I didn't understand what you said here. You mean that if template preparation is OK but the loading itself is bad (due to bubbles, for example), I should expect little...
Forum: Ion Torrent 11-30-2016, 10:16 AM
Replies: 9
Views: 4,530
Posted By lucorum
r.rosati, thanks a lot! I should try your...

r.rosati, thanks a lot!

I should try your method of storing used chips ("the opposite procedure" here means 2x isopropanol -> 2x flush buffer-> 3x 100 ul of 50% annealing buffer, am I right?) Do...
Forum: Ion Torrent 11-29-2016, 05:59 AM
Replies: 9
Views: 4,530
Posted By lucorum
Poor Ion PI Chip loading - can it possibly be caused by some contamination?

Hello everyone,
I have been working with Ion Torrent Proton sequencer for almost two years now. I do an RNA-Seq pipeline: prepare cDNA libraries (Ion Total RNA-Seq Kit v2), then perform emulsion PCR...
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