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Search: Posts Made By: Patrick
Forum: Bioinformatics 02-15-2010, 03:02 PM
Replies: 8
Views: 3,584
Posted By Patrick
Hi, I just subscribe with the AMOS help. I...

Hi,

I just subscribe with the AMOS help. I learning from there now. What problem you have facing now? Anything I can help you too? Maybe we face the same situation too.
Forum: 454 Pyrosequencing 10-16-2009, 06:46 PM
Replies: 0
Views: 2,583
Posted By Patrick
MIRA:454 scaffolds polishing by Illumina reads discussion

I got a bacteria genome which sequencing by 454 technique and Illumina technique separately.
After run 454 reads by using MIRA and BAMBUS, I extract the longest scaffold and treat it as a backbone....
Forum: Bioinformatics 09-13-2009, 05:23 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi BENM, Thanks for your suggestion. The...

Hi BENM,

Thanks for your suggestion. The knowledge is very useful for my research in future.
Do you got read the YH project as well?
I just read it last week and found out it is quite...
Forum: Illumina/Solexa 09-13-2009, 05:07 PM
Replies: 6
Views: 5,691
Posted By Patrick
Hi ScottC, Really thanks a lot for your...

Hi ScottC,

Really thanks a lot for your suggestion.
I understand what I should do now.
Thanks a lot ya :)
Hope that we can share our knowledge in future ^^
Forum: Illumina/Solexa 09-13-2009, 04:50 PM
Replies: 6
Views: 5,691
Posted By Patrick
Hi ScottC, Thanks for your suggestion. I...

Hi ScottC,

Thanks for your suggestion. I appreciate it :)
For your opinion, normally what control lane will use for human genome process sequencing by Illumina/Solexa?
Thanks again :p
Forum: Bioinformatics 09-11-2009, 03:38 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi Henry, You are right. I got read "improved...

Hi Henry,
You are right. I got read "improved base calling for the illumina genome analyzer using machine learning strategies" as well :)
It is quite useful for me to understand more about the...
Forum: Bioinformatics 09-11-2009, 12:39 AM
Replies: 26
Views: 8,544
Posted By Patrick
Hi henry, Do you have experience deal with...

Hi henry,
Do you have experience deal with Illumina/Solexa data?
Do you know what is the general control lane used by them among total eight lane produced by them?
Thanks a lot for your...
Forum: Bioinformatics 09-10-2009, 07:19 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi henry, Glad to know you at this forum :)

Hi henry,
Glad to know you at this forum :)
Forum: Bioinformatics 09-10-2009, 12:44 AM
Replies: 6
Views: 4,058
Posted By Patrick
Thanks for your reply... What you mention,make...

Thanks for your reply...
What you mention,make senses too...
I will try to find out more about the "unique mapped read" and share it with everybody :)
Forum: Bioinformatics 09-09-2009, 05:44 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi henry, I very thanks and appreciate for...

Hi henry,

I very thanks and appreciate for your info.
I learn a lot from you :)
To search for potential breast carcinoma susceptibility genes, I maybe will try to use the mgrc company free...
Forum: Bioinformatics 09-09-2009, 05:00 PM
Replies: 6
Views: 4,058
Posted By Patrick
Thanks a lot, westerman. Your reply makes me...

Thanks a lot, westerman.
Your reply makes me more understanding about how the scientist analyze the data.

Sad to said that I still not very clear about why the scientist will use the uniquely...
Forum: Bioinformatics 09-09-2009, 04:46 PM
Replies: 26
Views: 8,544
Posted By Patrick
For example: "In particular, carriers of the...

For example: "In particular, carriers of the breast cancer susceptibility genes, BRCA1 and BRCA2, are at a 30-40% increased risk for breast and ovarian cancer, depending on in which portion of the...
Forum: Bioinformatics 09-09-2009, 03:03 AM
Replies: 26
Views: 8,544
Posted By Patrick
Hi, Thanks a lot for your concern. I just...

Hi,

Thanks a lot for your concern.
I just try to link the breast cancer research with the available online bioinformatics tools.
My supervisors want me to use the available online bioinformatics...
Forum: Bioinformatics 09-08-2009, 11:51 PM
Replies: 6
Views: 4,058
Posted By Patrick
Hi simon, Thanks a lot for your suggestion....

Hi simon,

Thanks a lot for your suggestion. It is very clear and easy to understand.
Thanks for helping me solved my doubts :)
In your opinion, the definition for the uniquely mapped reads that...
Forum: Bioinformatics 09-08-2009, 10:25 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi Jing, Glad to hear that you research on...

Hi Jing,

Glad to hear that you research on oncogenesis now.
If I got any latest info about it, sure will told you d!
I recently focus on the research of breast cancer and related it with...
Forum: Bioinformatics 09-08-2009, 06:13 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi, Jing. You are right. "All people can...

Hi, Jing.

You are right. "All people can benefit from communications".
Hope that we can share a lot of experiences in processing and analyzing data in future.
Normally, what fields you prefers...
Forum: Bioinformatics 09-08-2009, 04:34 AM
Replies: 26
Views: 8,544
Posted By Patrick
Hi, Henry. Never mind. I will try to find out...

Hi, Henry.
Never mind. I will try to find out the soft-copy of the paper from the author.
Anywhere thanks a lot for your sharing.
I'm appreciate it.
Hope that we can share a lot of bioinformatics...
Forum: Bioinformatics 09-07-2009, 07:53 PM
Replies: 26
Views: 8,544
Posted By Patrick
Hi, Henry. Thanks for sharing. Do you have...

Hi, Henry.

Thanks for sharing.
Do you have the softcopy of the " structural variation in the human genome" paper?
I got try do download it from internet.
Unfortunately, I only can read the...
Forum: Bioinformatics 09-05-2009, 06:08 PM
Replies: 26
Views: 8,544
Posted By Patrick
Structural variation in human genome...

Can anybody briefly explain the general info about the structural variation in human genome?
Thanks a lot for sharing information :)
Forum: Bioinformatics 09-05-2009, 05:59 PM
Replies: 4
Views: 2,593
Posted By Patrick
Thanks a lot for your suggestion. Hopefully I can...

Thanks a lot for your suggestion. Hopefully I can get my desired output result and detail of breast cancer and bioinformatics :)
Forum: Bioinformatics 09-04-2009, 02:06 AM
Replies: 8
Views: 3,584
Posted By Patrick
AMOS & BAMBUS program experience sharing and general user guide.

Hi,

Anybody willing to share the experience of using AMOS & BAMBUS program?
Can give the general explanation about this two program and general user guide of these two program?
Thanks a lot for...
Forum: Bioinformatics 09-04-2009, 01:40 AM
Replies: 4
Views: 2,593
Posted By Patrick
Breast cancer research papers or articles links to bioinformatics...

Hi,
Anyone willing to share some experience or knowledge about the breast cancer research papers or articles that links to bioinformatics?
Thanks a lot for your advise and experience.
I will...
Forum: De novo discovery 06-23-2009, 06:42 PM
Replies: 0
Views: 3,089
Posted By Patrick
De novo short read assembly? Which assembler is the best?

Hi,
I just learn the short read assembly. I know got few assembler program such as velvet, mira and edena, etc are available now.
Got anybody got experience using those three assembly for short...
Forum: Bioinformatics 03-29-2009, 04:30 PM
Replies: 2
Views: 3,342
Posted By Patrick
Hi, thanks a lot. I finally solve this problem...

Hi, thanks a lot.
I finally solve this problem already. Really thanks for your advise.:)
Forum: Bioinformatics 03-20-2009, 12:11 AM
Replies: 2
Views: 3,342
Posted By Patrick
Glimmer3.0 problem...

Hi,
Glimmer is a collection of programs for identifying genes in microbial DNA sequences.
For running the glimmer requires 4 steps:
1. long-orfs USAGE: ./long-orfs <genome-file> [options]
2....
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