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Forum: Ion Torrent 03-09-2013, 07:02 AM
Replies: 4
Views: 3,133
Posted By RonanC
Anyone else had enough of Life Technologies?

I need to know if anyone else out there is as annoyed as I am about the whole 300/400 bp sequencing/OneTouch 2 fiasco?

We were led to believe that we would be able to do 300/400 bp sequencing when...
Forum: Ion Torrent 03-06-2013, 11:21 AM
Replies: 9
Views: 3,654
Posted By RonanC
I am doing RNA-seq on bacterial RNA samples and...

I am doing RNA-seq on bacterial RNA samples and I'm having terrible problems with rRNA contamination. Up to 90% of my reads are rRNA!

Currently I am using the Ambion MicrobExpress kit to remove...
Forum: Ion Torrent 07-11-2012, 10:37 AM
Replies: 2
Views: 5,960
Posted By RonanC
There's a thread over on the ion community board...

There's a thread over on the ion community board about this and it seems to me like people are not getting great results back from their paired end runs. IonTorrent are supposed to be coming out...
Forum: Ion Torrent 07-11-2012, 10:30 AM
Replies: 5
Views: 5,879
Posted By RonanC
When you do your initialization you have to have...

When you do your initialization you have to have an old chip in place in the PGM. Make sure that the chip in place is NOT a chip that you have used for a chlorite cleaning run. If it has been used...
Forum: Ion Torrent 07-05-2012, 12:50 PM
Replies: 27
Views: 18,974
Posted By RonanC
I just noticed that CLC bio are offering a free 6...

I just noticed that CLC bio are offering a free 6 month trial of their CLC genomics workbench to users with a benchtop NGS (i.e. 454 GS Jr, MiSeq or IonTorrent PGM). Anybody have any experience with...
Forum: Ion Torrent 07-05-2012, 10:43 AM
Replies: 4
Views: 3,344
Posted By RonanC
Hey there, Out of curiosity why have you...

Hey there,

Out of curiosity why have you decided to drop the ion torrent? We just got one so I'm curious to see what you didn't like about it?

Thanks,

Ronan.
Forum: Ion Torrent 07-05-2012, 10:20 AM
Replies: 2
Views: 6,486
Posted By RonanC
Ion Torrent Data Analysis

Hi everyone,

I wanted to start a thread to get an idea what other ion torrent users are using/doing for their downstream data analysis. There are a number of preferred software vendors suggested...
Forum: Introductions 07-03-2012, 11:34 AM
Replies: 1
Views: 998
Posted By RonanC
New Ion Torrent user in Florida

Hi everyone,

I'm a postdoc microbiologist and we just purchased an IonTorrent PGM. It is my responsibility to get the thing up and running and turn the data into something that makes sense to...
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