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Search: Posts Made By: silasysh
Forum: Bioinformatics 12-23-2014, 03:59 AM
Replies: 2
Views: 2,343
Posted By silasysh
Thank you for your reply. At least the package...

Thank you for your reply. At least the package v2.5 is still free. I found the current database becomes b2g_sep13. Now everything is okay.

Silas
Forum: Bioinformatics 12-22-2014, 03:54 AM
Replies: 2
Views: 2,343
Posted By silasysh
Blast2go pipeline

dear all,

I have two questions about blast2go.

1. Is the blast2go pipeline(b2g4pipe) still free to use. Because I checked the website of Blast2Go. The command line version need to buy. Are...
Forum: Bioinformatics 09-18-2014, 01:08 AM
Replies: 2
Views: 1,098
Posted By silasysh
Thank you. If I just do the following: ...

Thank you.

If I just do the following:

samtools mpileup -ugf my.fasta my.bam

the result is like what I posted before. Nothing more.

I will try to post bam file to samtools-help email...
Forum: Bioinformatics 09-17-2014, 02:27 PM
Replies: 2
Views: 1,098
Posted By silasysh
Samtools call variant

Hi,

I used samtools to call variant. I use the latest version 1.0. First, I have to generate bcf file. I run the following command.

samtools mpileup -t DP -ugf my.fasta my.bam

the result is...
Forum: Bioinformatics 08-29-2014, 02:05 AM
Replies: 0
Views: 1,325
Posted By silasysh
How to change track color by using batch script of IGV

Dear all,

Recently, I have to generate many screenshots of IGV. therefore, I am trying to use batch script to achieve it. Because I have a lot of libraries, I want to
change the track color for...
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