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Forum: Illumina/Solexa 08-20-2014, 07:27 AM
Replies: 2
Views: 1,936
Posted By hduborjal
PCR directly on DNA binds to Ampure XP

Hi everybody,
During the library preparation it's possible to conserved Ampure beads during the different enzymatic reaction (it's the with beads strategy for improving yield, Fisher et al. Genome...
Forum: Illumina/Solexa 07-07-2014, 09:53 PM
Replies: 3
Views: 3,576
Posted By hduborjal
Hi, thank you for your answer. I understand...

Hi, thank you for your answer.
I understand that you use dual indexing.
My first question is : Did you try specific Xgen blocker or the universal one for Illumina HT libraries ?
My second one :...
Forum: Illumina/Solexa 03-21-2014, 06:06 AM
Replies: 5
Views: 1,701
Posted By hduborjal
Methylated adaptor are protected from bisulflite...

Methylated adaptor are protected from bisulflite treament which converts unprotected cytosine to thymine.
If you want to carry out a WGBS project, you can produce library with methylated adpator....
Forum: Illumina/Solexa 03-21-2014, 01:48 AM
Replies: 3
Views: 3,576
Posted By hduborjal
test of Xgen blocking oligo

Did someone try the XGen blocking oligo from IDT instead of classical blocking oligos ? Is there a improvement of the off target ?
Forum: Illumina/Solexa 03-21-2014, 01:42 AM
Replies: 5
Views: 1,701
Posted By hduborjal
Hi, Methylated adpators are just protected from...

Hi,
Methylated adpators are just protected from Bisulfite conversion. By default Illumina's adaptors from low throughput kit are methylated. There is no negative effect on the library. It can be use...
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