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Forum: Illumina/Solexa 12-07-2017, 03:35 PM
Replies: 9
Views: 992
Posted By clseq2017
I agree with you! But Illumina looked at the run...

I agree with you! But Illumina looked at the run and they said the parameters are still acceptable. However, I want to repeat and fix this overclustering issue. But my questions are : how do I avoid...
Forum: Illumina/Solexa 12-07-2017, 08:52 AM
Replies: 9
Views: 992
Posted By clseq2017
Nucleotide Diversity

Hello,

You mentioned "low diversity" library but looking at some troubleshooting guidelines it looks like my library is unbalanced (see attached picture).
What is an unbalanced library? How can I...
Forum: Illumina/Solexa 12-07-2017, 07:34 AM
Replies: 9
Views: 992
Posted By clseq2017
Hello, The cluster density was very high 1600...

Hello,
The cluster density was very high 1600 K/mm2 (supposed to be 1000-1200 K/mm2 for V2 reagents).
Yes, I have had better runs but the cluster density has always been in the upper limit. I am...
Forum: Illumina/Solexa 12-06-2017, 11:05 AM
Replies: 9
Views: 992
Posted By clseq2017
OverClustering - MiSeq v2 reagents - Nextera XT Library Prep

Hi everyone,

I am a new user of MiSeq. I am using the Nextera XT Library Prep for sequencing of gDNA along with the MiSeq Reagents v2.

I am having issues with overclustering, and the Q30 was...
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