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Forum: Bioinformatics 10-30-2019, 01:37 PM
Replies: 3
Views: 1,802
Posted By radood
Wow I do see it now!!! Thank you so very much...

Wow I do see it now!!! Thank you so very much Rosati. Yes this is super helpful and it makes a lot of sense :) :) :)

I didn't realize that reads in the SAM file always represent the forward...
Forum: Bioinformatics 10-28-2019, 12:27 PM
Replies: 3
Views: 1,802
Posted By radood
Checking to see if folks have feedback on this. I...

Checking to see if folks have feedback on this. I read that "BWA actually follows the SAM spec and reports Phred scores as MAPQ values.* The calculation is based on the number of optimal (best)...
Forum: General 10-24-2019, 12:56 PM
Replies: 6
Views: 2,901
Posted By radood
You could try contacting the company that...

You could try contacting the company that synthesized them originally (idt, sigma, etc..) provided you still have some identifier on the tube to ask if they will forward you the information. That...
Forum: Bioinformatics 10-24-2019, 08:19 AM
Replies: 3
Views: 1,802
Posted By radood
Question Wrong bwa alignment?

Hi,
Below is a supplementary alignment for one of my reads with a mapping quality score of 43.This should map to chr2: 29446797 in hg19
...
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