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Forum: Sample Prep / Library Generation 11-22-2017, 10:50 AM
Replies: 4
Views: 1,833
Posted By ericchow
It's a result of the 28S from insects being...

It's a result of the 28S from insects being split. See this article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016993/
Forum: Illumina/Solexa 01-11-2016, 11:32 AM
Replies: 31
Views: 11,534
Posted By ericchow
There's a YouTube video:...

There's a YouTube video: https://www.youtube.com/watch?v=JBmOWNmj4Gk

From the video, the flow cell is fairly large. The sequencer screen shows a cluster density of 196K/mm2, similar to the...
Forum: Sample Prep / Library Generation 05-26-2015, 10:44 AM
Replies: 5
Views: 2,739
Posted By ericchow
Can you decrease the aspiration and dispense...

Can you decrease the aspiration and dispense rates to lower the pressure?

Are these tips new each time or are they resused from run to run since they're metal? Can you pipet some of the Tn mix...
Forum: Illumina/Solexa 01-21-2014, 10:15 AM
Replies: 100
Views: 41,087
Posted By ericchow
According to the NextSeq spec sheet...

According to the NextSeq spec sheet (http://res.illumina.com/documents/products/datasheets/datasheet-nextseq500.pdf), in addition to the 520 and 650nm LEDs, it also has a 780nm laser. At first I...
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