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Forum: Oxford Nanopore 07-12-2019, 04:27 AM
Replies: 0
Views: 877
Posted By Femta
Circulomics Size Selection

Hi Community,

I tried today for the first time the Circulomics Size Selection Kit with DNA for a library for MinION.
I started with 5 g DNA with known size (above 40kb, but with fragments...
Forum: Oxford Nanopore 04-02-2019, 06:31 AM
Replies: 3
Views: 982
Posted By Femta
Hi, well you initially asked why there are...

Hi,

well you initially asked why there are these small fragments ;-) And since you didn't see them in your QC before the library prep it's most likely that that happen due to pipetting during the...
Forum: Oxford Nanopore 03-28-2019, 04:57 AM
Replies: 3
Views: 982
Posted By Femta
Hi, as far as I've seen you have clean up...

Hi,

as far as I've seen you have clean up steps in LSK308. These steps are risky to fragment your DNA.
So try to avoid as many as possible to pipet your DNA during the library preparation. To...
Forum: Oxford Nanopore 10-12-2018, 03:09 AM
Replies: 2
Views: 1,797
Posted By Femta
PromethION and Barcoding

Dear all,

we will get a PromethION machine surprisingly soon, and you know, rumours are fast, so all of our researchers are already standing in our lab to have something sequenced.

The...
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