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Forum: Core Facilities 09-12-2019, 02:02 PM
Replies: 5
Views: 4,677
Posted By capsicum
We use Microsoft Surface tablets. They're...

We use Microsoft Surface tablets. They're powerful enough to do pretty much anything we'll need to do at the lab bench, they have good connectivity, and ease of use and application support is also...
Forum: MGISEQ (FKA Complete Genomics) 09-12-2019, 01:44 PM
Replies: 2
Views: 5,406
Posted By capsicum
For what it's worth (there's very littel traffic...

For what it's worth (there's very littel traffic here anyway):

I think this should be renamed back to Complete Genomics, and a new forum should be created for MGISEQ instruments. Most of the...
Forum: Metagenomics 03-16-2014, 02:23 PM
Replies: 3
Views: 2,193
Posted By capsicum
Yes, but how reliable do you normally find them?...

Yes, but how reliable do you normally find them? Some people must find them reliable enough to perform both at the same time (in the same reaction). I'd be worried that the product from the 1st PCR...
Forum: Metagenomics 03-13-2014, 09:10 PM
Replies: 3
Views: 2,193
Posted By capsicum
Illumina 16S libs - tech & QC questions about construction and data comparison

I wanted to ask for people's opinions and experiences:

I'm looking at making some Illumina-compatible sequencing libraries. Illumina has a two-step PCR method where the first PCR amplifies a...
Forum: Sample Prep / Library Generation 02-12-2013, 12:42 PM
Replies: 0
Views: 818
Posted By capsicum
Metatranscriptomics - RNA gel image

Hello,

Can anyone post a picture of their RNA input to a metatranscriptomics experiment?

Thanks.
Forum: Metagenomics 11-30-2012, 11:48 PM
Replies: 15
Views: 10,240
Posted By capsicum
Hi Andy, Are you using a V2 or V2 MiSeq?...

Hi Andy,

Are you using a V2 or V2 MiSeq? Which kit versions are you using? What yields do you get and what are the quality metrics like?

Cheers,

Scott.
Forum: Metagenomics 11-21-2012, 01:10 PM
Replies: 0
Views: 2,105
Posted By capsicum
Who uses a MiSeq for 16S data?

Hi Everyone,

I'm interested to hear about anyone's experiences with 16S sequencing on the MiSeq. I'm aware of the papers by Caporaso, Illumina's tech notes, methods for 'hard-coding' matrix and...
Forum: Illumina/Solexa 10-29-2012, 01:18 PM
Replies: 28
Views: 13,081
Posted By capsicum
What cluster density are you getting on these...

What cluster density are you getting on these runs?
Forum: Metagenomics 10-23-2012, 09:35 PM
Replies: 15
Views: 10,240
Posted By capsicum
Hi Nick, How much difference do you see when...

Hi Nick,

How much difference do you see when hardcoding the matrix and phasing values? Illumina is telling me not to expect anything significant.
Forum: Illumina/Solexa 10-22-2012, 05:07 PM
Replies: 28
Views: 13,081
Posted By capsicum
So things have gotten worse since the hardware...

So things have gotten worse since the hardware upgarde? What has caused this... hardware or software? It sounds like just a software issue (well, perhaps a methodological issue and that methodology...
Forum: Illumina/Solexa 10-22-2012, 04:15 PM
Replies: 28
Views: 13,081
Posted By capsicum
Can anyone share some run statistics for 16S runs...

Can anyone share some run statistics for 16S runs on the MiSeq? What cluster density are you aiming for? What PhiX spike-in proportion are you using? What cluster density, sequence yield and sequence...
Forum: Metagenomics 07-02-2012, 04:33 PM
Replies: 3
Views: 2,685
Posted By capsicum
Are there any publications for this software? Any...

Are there any publications for this software? Any test data?
Forum: Metagenomics 07-02-2012, 04:26 PM
Replies: 6
Views: 4,146
Posted By capsicum
I think they're using V4 now for Illumina...

I think they're using V4 now for Illumina sequencing.
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