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Forum: Sample Prep / Library Generation 07-19-2017, 10:43 PM
Replies: 2
Views: 913
Posted By wangwde
Thanks! Swift Bio for example using this...

Thanks!
Swift Bio for example using this strategy to build their Libraries, for DNAseq and RNAseq. It should be different for DNA and RNA application, right?
Forum: Sample Prep / Library Generation 07-19-2017, 02:03 AM
Replies: 2
Views: 913
Posted By wangwde
How the sequential adaptation process works?

Hi, saw some library kits using sequential adatation process, first to add adapter to the 5 ' prime and then 3'prime, just wondering how it works, many thanks in advance!
Forum: RNA Sequencing 03-20-2017, 04:31 AM
Replies: 2
Views: 1,864
Posted By wangwde
Thanks Phillip, I do think that was just maschine...

Thanks Phillip, I do think that was just maschine error and I have proceeded the library construction without other attemptions, hopefully it works.
Forum: RNA Sequencing 03-10-2017, 07:03 AM
Replies: 2
Views: 1,864
Posted By wangwde
28s RNA peak is missing in Human RNA

Dear all,
Please see attached pic of human total RNA analyzed by Fragment analyzer, the 28s rRNA peak is somehow missing (blue peak), any idea? it seems not degraded...
Thank you for you help.
Forum: Illumina/Solexa 02-15-2016, 04:02 AM
Replies: 2
Views: 1,261
Posted By wangwde
Difference when shearing with Low-EDTA and Water?

Dear all,
Does anyone have ever test the shearing protocol provided by Covaris with different buffer? e.g. low-EDTA buffer and Water?
thank you!
Wei
Forum: Sample Prep / Library Generation 09-24-2014, 02:15 AM
Replies: 9
Views: 3,492
Posted By wangwde
Hi, did you shear your FFPE gDNA using covaris?...

Hi, did you shear your FFPE gDNA using covaris? In my opinion, even the FFPE DNA was degraded, it is still way more larger than the shared DNA fragments we used for WGS library construction.
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