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Forum: Pacific Biosciences 11-04-2014, 09:08 PM
Replies: 0
Views: 1,454
Posted By aliceli
ligation problem for pcr product

We have 10k pcr product to construct the pacbio library. The original and concentrate DNA look good on bioanalyzer, and the band is pure 10kb band. However, the library is smear from 3kb to 10kb....
Forum: Sample Prep / Library Generation 11-28-2012, 07:52 AM
Replies: 0
Views: 1,210
Posted By aliceli
NuGEN OvationŽ RNA‑Seq with PacBio sequencing

What is the product by using NuGEN OvationŽ RNA‑Seq?
ss-cDNA or ds-cDNA? Only ds-cDNA can be made into successful PacBio library, so that is very important message.
Does anyone have experience on...
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