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Forum: Illumina/Solexa 05-21-2015, 08:24 PM
Replies: 4
Views: 2,800
Posted By nucacidhunter
I have not done exact math. Using 10 uM (each) 40...

I have not done exact math. Using 10 uM (each) 40 base long adapter oligos with full complimentary bases across oligos I get around 70 ng/ul with PicoGreen and for 10 uM 30 base long oligos with 14...
Forum: Illumina/Solexa 05-21-2015, 04:18 PM
Replies: 4
Views: 2,800
Posted By nucacidhunter
As you have done, Qubit or PicoGreen is the...

As you have done, Qubit or PicoGreen is the easiest and very informative. With increasing annealing efficiency you should see increasing value in concentration of dsDNA. I regularly use this method...
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