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Forum: Sample Prep / Library Generation 04-12-2017, 10:12 AM
Replies: 12
Views: 2,669
Posted By pmiguel
No idea why the Zymo columns are cheaper. They...

No idea why the Zymo columns are cheaper. They work well. We use them sometimes.

The DNAse/RNA clean-up ones work too. Although people complain of yield issues. Some of the qiagen columns allow...
Forum: Sample Prep / Library Generation 04-12-2017, 08:28 AM
Replies: 12
Views: 2,669
Posted By pmiguel
DNAse itself should not degrade RNA as long as it...

DNAse itself should not degrade RNA as long as it it pure. But if your samples have residual RNAses in them from the cells from which they derive, the during the DNAse incubation, those RNAses may...
Forum: Sample Prep / Library Generation 04-12-2017, 02:55 AM
Replies: 12
Views: 2,669
Posted By pmiguel
Okay, at this juncture, your real problem is that...

Okay, at this juncture, your real problem is that you don't know what concentration your RNA is actually at. Secondarily you have some Trizol (both phenol and some guanidium salt at 230nm) remaining...
Forum: Sample Prep / Library Generation 04-11-2017, 12:14 PM
Replies: 12
Views: 2,669
Posted By pmiguel
Trizol is an excellent source of phenol...

Trizol is an excellent source of phenol contamination.
I'm sure you understand that measuring the absorbance of a sample at 260nm in no way proves that that absorbance derives from nucleic acids,...
Forum: Sample Prep / Library Generation 04-11-2017, 04:42 AM
Replies: 12
Views: 2,669
Posted By pmiguel
Hi Renuka22, You are giving us almost no...

Hi Renuka22,
You are giving us almost no information to work with -- for instance what assay told you that you have 200-300 ng/ul? What is your source of RNA (organism and tissue)? What type of...
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