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Search: Posts Made By: anle
Forum: Bioinformatics 08-24-2012, 10:07 AM
Replies: 0
Views: 1,035
Posted By anle
Helicos sequencing analysis

Hi,
I have downloaded a set of sra files from Helicos sequencing data and I wonder hw to proceed analyzing them. I found these tools provided from the company...
Forum: Bioinformatics 01-25-2012, 01:25 PM
Replies: 0
Views: 1,804
Posted By anle
small rna seq with total lane read count different between conditions

I have a control and a knockdown experiment from which I am sequencing small rnas. When I count the sum of the reads mapped to the known mirnas foreach condition I get 968384 for contol anD 71736 for...
Forum: Bioinformatics 01-16-2012, 12:24 AM
Replies: 0
Views: 1,211
Posted By anle
RNA seq with two time points

Hi,
I wanna analyze RNA-seq data of two time points (each one has a control and a knocked down sample) in order to find genes that are constitutionally down or up regulated. i was thinking of...
Forum: RNA Sequencing 12-22-2011, 05:14 AM
Replies: 24
Views: 11,317
Posted By anle
Thnx!! Sorry I was misconfused but...

Thnx!! Sorry I was misconfused but marginalization and conditional probabilities make me think of Bayes
Forum: RNA Sequencing 12-22-2011, 04:30 AM
Replies: 24
Views: 11,317
Posted By anle
Hi Simon I have some things confused in mmy mind...

Hi Simon I have some things confused in mmy mind and I would need some help
You say:
"Let Q be a random variable with gamma distribution with expectation q0 and variance a q0"

If I understand...
Forum: Bioinformatics 05-24-2011, 10:55 PM
Replies: 0
Views: 1,319
Posted By anle
Question Confused with sam flags

I have a paired end rna sequence alignment. I have a pair of reads that one has the flag 103 and the other 147
103 means:
read paired
read mapped in proper pair
read unmapped
mate reverse...
Forum: Bioinformatics 05-11-2011, 02:22 PM
Replies: 2
Views: 4,022
Posted By anle
RNA seq analysis with one replicate per biological sample

Hi, I am new in the field of deep sequencing analysis and for the past two months I am struggling to understand RNA seq differential expression studies.
I have 1 control sample and 1 sample for...
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