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Forum: Bioinformatics 11-28-2015, 02:48 PM
Replies: 136
Views: 46,475
Posted By bluepoison
Hi Felix, Thanks a lot for quick response....

Hi Felix,

Thanks a lot for quick response. It was really helpful for me.

I just performed a short experiment. Just wanted to share with you. I randomly pooled 1M reads, and made 3 following...
Forum: Bioinformatics 11-28-2015, 08:46 AM
Replies: 136
Views: 46,475
Posted By bluepoison
Hi all, This is my first sequencing data...

Hi all,

This is my first sequencing data analysing. I am having difficulties trimming the adapters/contaminants from the reads. I have got 50bp single paired read. I checked in fastqc that there...
Forum: Bioinformatics 11-26-2015, 03:11 PM
Replies: 5
Views: 4,246
Posted By bluepoison
Thanks a lot for the response Brian. I have...

Thanks a lot for the response Brian.

I have single reads this time. Do you have any suggestions for the overrepresented sequences that do not match with any actual adapter (''No Hit" as described...
Forum: Bioinformatics 11-22-2015, 07:02 PM
Replies: 5
Views: 4,246
Posted By bluepoison
finding adapters for trimming

Hi All,

I am a total newbies in this field. I want to know the trend of the community for adapter trimming steps.

I have got 50bp single end reads (Sanger / Illumina 1.9). Primary goal is to...
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