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Forum: Epigenetics 12-28-2015, 06:24 AM
Replies: 6
Views: 2,788
Posted By Mkrause
Thank you all for your replies! Would you...

Thank you all for your replies!

Would you think that using paired-end seq might help?
First of all it is likely that one of the two reads is not multi-mapper, and thus would give more confidence...
Forum: Epigenetics 12-28-2015, 05:05 AM
Replies: 6
Views: 2,788
Posted By Mkrause
That one single read maps to more than one...

That one single read maps to more than one position in the genome with the same confidence.

Is there any other definition?
Forum: Epigenetics 12-28-2015, 02:26 AM
Replies: 6
Views: 2,788
Posted By Mkrause
Yes sure. IT makes 10-20% of the whole genome....

Yes sure.
IT makes 10-20% of the whole genome. However, in next experiments I do not want to waste 80% of my data on multimappers, if avoidable.

As the problem appears also in the Input, I...
Forum: Sample Prep / Library Generation 11-17-2015, 08:08 AM
Replies: 0
Views: 596
Posted By Mkrause
What library fits best for MNase ChIP experiments

Hello Community.
I am facing problems with mappability after doing MNase ChIP-Seq.
Somehow I enrich for multimapper fraction in Seq results.
The quality and complexity of the reads are fine, but...
Forum: Epigenetics 11-10-2015, 02:32 PM
Replies: 6
Views: 2,788
Posted By Mkrause
Mnase cause high multimapper fraction?

Hello Community.

I am working on histone modifications in zebrafish.
I recently ran several samples for a histone methylation ChIP after MNase digestion.

Now I run into the problem of having...
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