Forum: Sample Prep / Library Generation
12-21-2017, 11:26 AM
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Replies: 8
Views: 2,723
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Forum: RNA Sequencing
11-12-2017, 04:55 PM
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Replies: 5
Views: 2,456
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Forum: Pacific Biosciences
09-28-2017, 03:30 AM
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Replies: 62
Views: 29,147
One library, primer annealing, polymerase...
One library, primer annealing, polymerase binding, clean up and Magbead binding was done in one batch (one tube) then was splitted into three wells on the same sample plate and sequenced using the...
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Forum: Pacific Biosciences
09-28-2017, 01:25 AM
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Replies: 62
Views: 29,147
We have found Sequel output to be inconsistent...
We have found Sequel output to be inconsistent and not sure if it is our machine. Just completed three SMRT cell runs with one library prepared for sequencing in bulk sequenced one after another but...
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Forum: Pacific Biosciences
09-03-2017, 02:52 PM
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Replies: 7
Views: 3,166
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Forum: Illumina/Solexa
06-06-2016, 08:20 PM
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Replies: 13
Views: 4,890
Thanks for reply Mike. I am not clear at the...
Thanks for reply Mike. I am not clear at the start of which read (R1, R2 or both) the inline barcode will be sequenced, if inline barcode is added to 5’ end of forward primer. The structure of...
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Forum: Illumina/Solexa
06-06-2016, 04:41 PM
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Replies: 13
Views: 4,890
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Forum: Core Facilities
05-04-2016, 06:48 PM
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Replies: 8
Views: 8,501
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Forum: Core Facilities
05-04-2016, 02:33 AM
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Replies: 8
Views: 8,501
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Forum: Sample Prep / Library Generation
04-17-2016, 11:06 PM
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Replies: 10
Views: 3,697
There is some anecdotal evidence that libraries...
There is some anecdotal evidence that libraries prepared with this kit in input range (250pg-10ng) do not show any correlation with 100 ng or higher input libraries prepared with the same input total...
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Forum: Illumina/Solexa
07-23-2015, 07:21 PM
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Replies: 2
Views: 5,262
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Forum: Illumina/Solexa
07-22-2015, 05:51 PM
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Replies: 2
Views: 5,262
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Forum: Bioinformatics
06-23-2015, 02:48 AM
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Replies: 7
Views: 4,012
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Forum: Bioinformatics
06-21-2015, 01:44 AM
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Replies: 7
Views: 4,012
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Forum: Bioinformatics
06-19-2015, 02:16 AM
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Replies: 7
Views: 4,012
PacBio vs MinION
To compare PacBio long reads with MinION for de novo assembly of bacterial genomes what one should consider for library prep and assembly.
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Forum: Illumina/Solexa
11-22-2014, 02:53 AM
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Replies: 18
Views: 7,874
Thanks for clarifying your position on PCR...
Thanks for clarifying your position on PCR contamination issues. I do not think that a mechanism or evidence has been provided for PCR contamination you have implied in earlier posts:
I was...
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Forum: Illumina/Solexa
11-22-2014, 12:05 AM
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Replies: 18
Views: 7,874
If I understand you correctly, you are saying...
If I understand you correctly, you are saying that contamination by PCR is inevitable and nothing can be done about it. I have to disagree with you because PCR has been and is and probably will be...
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Forum: Bioinformatics
09-30-2014, 01:06 PM
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Replies: 4
Views: 1,190
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Forum: Bioinformatics
09-29-2014, 11:27 PM
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Replies: 4
Views: 1,190
Mate pair distance calculation
Hi
I have two mate pair libraries with 2-3 kb and 6-8 kb inserts from a plant species. The libraries have been sequenced 2x100 cycles. FastQC shows good quality reads with low duplication levels....
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