SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 5 of 5
Search took 0.00 seconds.
Search: Posts Made By: klytie
Forum: Core Facilities 02-28-2012, 11:21 PM
Replies: 16
Views: 6,846
Posted By klytie
Thank you! I have already contacted...

Thank you!
I have already contacted KBiosciences in UK (sorry for not mentioning this before) but this was like two weeks ago and no answer so far. So I was wondering whether there was some other...
Forum: Core Facilities 02-28-2012, 10:14 AM
Replies: 16
Views: 6,846
Posted By klytie
Hi! Does anyone know is there Covaris shearing ...

Hi! Does anyone know is there Covaris shearing service provider somewhere in Europe also?
Would be very-very thankful for the information.
Forum: Illumina/Solexa 08-04-2011, 03:11 AM
Replies: 0
Views: 1,483
Posted By klytie
sequencing with specific primers

what kind of conditions should one bear in mind when designing custom sequencing primers (instead of using Illumina universal sequencing primers).
Also, is it possible to ligate or add flow cell...
Forum: Sample Prep / Library Generation 07-06-2011, 11:00 PM
Replies: 3
Views: 4,885
Posted By klytie
Thank you for your answer. The enzyme is DNAseI...

Thank you for your answer. The enzyme is DNAseI and following end repair comes as usual A-tailing with Klenow exo-. So...I'd better use those three enzymes just to be sure my reactions work?
Forum: Sample Prep / Library Generation 07-06-2011, 06:22 AM
Replies: 3
Views: 4,885
Posted By klytie
T4 kinase in end repair protocol

Hi!
I'm new in this, so forgive me if my question is stupid. Why do I need to add T4 PNK to the mixture of Klenow and T4 polymerase when repairing the ends of enzyme fragmented library. I'm trying...
Showing results 1 to 5 of 5

 


All times are GMT -8. The time now is 05:24 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO