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Forum: Bioinformatics 04-06-2017, 09:14 AM
Replies: 104
Views: 41,589
Posted By indapa
Hi Brian, Thanks for your reply. I tried...

Hi Brian,

Thanks for your reply. I tried using Spades and got similar results. After speaking with my experimental colleague, I think I may have a mixture of viruses rather than a single one. I...
Forum: Bioinformatics 04-04-2017, 10:23 AM
Replies: 104
Views: 41,589
Posted By indapa
Hi Brian, I have used tadpole to assemble my...

Hi Brian,

I have used tadpole to assemble my viral species of interest using MiSeq 2x150 PE reads with very good results. I've gotten contigs >100kb in length and then have been able to merge...
Forum: Bioinformatics 02-08-2017, 10:57 PM
Replies: 104
Views: 41,589
Posted By indapa
getting a single unitig from Tadpole

I'm new to de-novo assembly, so sorry for the naive questions. I used Tadpole for a viral de-novo assembly that had some novel genes inserted and it worked very well. I was able to get ~13 contigs...
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