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Search: Posts Made By: durandg
Forum: SOLiD 07-15-2013, 08:32 AM
Replies: 5
Views: 4,592
Posted By durandg
Our FAS told me that this "new version" of the...

Our FAS told me that this "new version" of the capture kit has redesigned probes and more probes hat before but she wasn't able to tell me whether it is discontinued or not.

Investigation...
Forum: SOLiD 07-08-2013, 06:59 AM
Replies: 5
Views: 4,592
Posted By durandg
Low exomic yeld after capture.

Dear all,

After having used the Targetseq Exome Capture kit for years, LifeTech provided us with a new version of the kit called Ion TargetSeq™ Exome Enrichment kit for the exome capture of the...
Forum: General 07-08-2013, 06:41 AM
Replies: 4
Views: 3,948
Posted By durandg
Dear Aan, Here are the settings we're...

Dear Aan,

Here are the settings we're using, on a Covaris S220; we usually got a peak size at 160bp within a range from 150bp to 180bp.


Peak power 175
Duty factor 10 100s ...
Forum: SOLiD 06-18-2013, 04:46 AM
Replies: 0
Views: 1,528
Posted By durandg
Absence or incomplete ligation for SOLID lib prep.

Hello all,

I'm making fragment librairies on a Solid 5500XL, using LifeTech protocols and standard kits) and i am experiencing troubles in my last lib preps: using mouse DNA, i have no ligation...
Forum: SOLiD 06-04-2012, 06:58 AM
Replies: 1
Views: 1,306
Posted By durandg
Hi Jimmy, We started last week our first...

Hi Jimmy,

We started last week our first run with 2 flowchips in parallel on a 5500XL sequencer and the run is supposed to last 18 days for the conditions we setup. For the same conditions, one...
Forum: General 05-17-2012, 09:26 AM
Replies: 4
Views: 4,109
Posted By durandg
Update the NGS map.

Dear James,

I would like to update the map with the machines we now have in our lab but i don't find the way to add a bookmark on the map and enter the information i want to add...

Is this...
Forum: Sample Prep / Library Generation 05-17-2012, 02:13 AM
Replies: 8
Views: 1,886
Posted By durandg
Hi ValeD, Looking forward from your news...

Hi ValeD,

Looking forward from your news with next results! I hope it'll help and you'll get rid of your problems.

Don't hesitate if i can help.
Cheers,

D.
Forum: General 05-17-2012, 02:10 AM
Replies: 4
Views: 3,948
Posted By durandg
Hi Hamid, Thank you a lot! It's really...

Hi Hamid,

Thank you a lot! It's really hard to get informations for this kind of samples that are a bit tricky to work with, even on Covaris, Life Tech or other NGS related websites.

I have...
Forum: Sample Prep / Library Generation 05-16-2012, 07:31 AM
Replies: 8
Views: 1,886
Posted By durandg
Hi ValeD, Thanks Lalan for the link to the...

Hi ValeD,

Thanks Lalan for the link to the other thread! On my point of view, this is miRNA (probalby not degraded RNA because in that case it won't be a "huge" pack of degraded RNA only from...
Forum: Sample Prep / Library Generation 05-15-2012, 09:44 AM
Replies: 8
Views: 1,886
Posted By durandg
Hi, Would you have a pdf of your "bad"...

Hi,

Would you have a pdf of your "bad" profile's Bioanalyzer runs? It could help to picture your problem and try to help.

Otherwise Agilent Techsupport or FAS can be a good help; it was the...
Forum: Sample Prep / Library Generation 05-15-2012, 09:40 AM
Replies: 0
Views: 2,121
Posted By durandg
Ideas for shearing and/or size-selected FFPE DNA?

Hello,

I want to perform exome sequencing on a SOLID 5500 with DNA extracted from FFPE.

After a Bioanalyzer run, i've seen -of course- a range of sizes up to Kb.
Even with doing a shearing...
Forum: General 05-15-2012, 03:11 AM
Replies: 4
Views: 3,948
Posted By durandg
Red face Shearing of FFPE extracted DNA.

Hello,

I want to perform exome sequencing on a SOLID 5500 with DNA extracted from FFPE.

After a Bioanalyzer run, i've seen -of course- a range of sizes up to Kb.
Even with doing a shearing...
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