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Forum: Sample Prep / Library Generation 05-10-2016, 06:38 PM
Replies: 69
Views: 42,127
Posted By Runuply
Hi paramita7 I am using frozen cartilage for...

Hi paramita7

I am using frozen cartilage for ATAC-seq. I use collagenase to dissociate the chondrocytes.
What kind of frozen tissue do you use?
Forum: Sample Prep / Library Generation 05-10-2016, 06:33 PM
Replies: 69
Views: 42,127
Posted By Runuply
Hi Wonghe did you solve the problem? What kind...

Hi Wonghe did you solve the problem?
What kind of frozen tissue do you use? I feel like the cell isolation process is most important.
Forum: Sample Prep / Library Generation 03-23-2016, 10:51 PM
Replies: 69
Views: 42,127
Posted By Runuply
Hi LOH Could you please tell us, what should be...

Hi LOH
Could you please tell us, what should be the factors to get large size libraries sizes over 1kb. Do you think it is under-tagmentation, cell number dependent?

Best


Runuply
Forum: Sample Prep / Library Generation 03-08-2016, 12:11 AM
Replies: 69
Views: 42,127
Posted By Runuply
Thanks Mantis. I did the AMpure , it looks...

Thanks Mantis.
I did the AMpure , it looks good.
By the way, may I know that, normally how much DNA can you get after lysis if you use 50K cells? Do you check the concentration before PCR...
Forum: Sample Prep / Library Generation 02-29-2016, 04:37 PM
Replies: 69
Views: 42,127
Posted By Runuply
Hi mantis, thanks for your reply. This is my 2nd...

Hi mantis, thanks for your reply. This is my 2nd test.
May I know that how do you get rid of the primer peak? Do you use something like Ampure purification method?
Forum: Sample Prep / Library Generation 02-28-2016, 10:42 PM
Replies: 69
Views: 42,127
Posted By Runuply
Hi guys, I also used that protocol for ATAC-seq...

Hi guys, I also used that protocol for ATAC-seq from frozen tissue, PCR amplification 5 cycles; and 7 cycles for qPCR(1 to 3rd of Max Fluorescent). It is a little hard for me to control the exact...
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