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Forum: Sample Prep / Library Generation 09-04-2019, 06:00 PM
Replies: 1
Views: 634
Posted By Kujin Kwon
More than half of Smart-seq2 data is TSO,ISPCR concatemers!

Hi all,
Last month, I made fixed tissue (5< RIN < 7) RNA-seq library using SMART-seq2 method. (I thought single cell library prep method is suitable for fixed sample because of low quantity of RNA...
Forum: Illumina/Solexa 07-30-2019, 07:44 PM
Replies: 0
Views: 679
Posted By Kujin Kwon
Apply "Nextera" enzyme for RNA-seq library

Hi all,
Recently I'm trying to make RNA-seq library using Nextera XT kit.
However, I noticed that kit shipping takes 1 month.
As I have Nextera kit (Maybe old one) which was used in ATAC-seq, I'd...
Forum: General 03-22-2019, 07:41 AM
Replies: 4
Views: 1,661
Posted By Kujin Kwon
Thank you for your suggestion. I'll try to...

Thank you for your suggestion.

I'll try to change elution buffer to 0.1xTE or Nuclease free water.
Forum: General 03-18-2019, 01:49 AM
Replies: 4
Views: 1,661
Posted By Kujin Kwon
Question Size selected library is not amplified

Hi all,

I'm trying to make RNA library using "NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina"
After final amplification, I found that dimers are found in library product by...
Forum: General 12-21-2018, 12:59 AM
Replies: 3
Views: 1,425
Posted By Kujin Kwon
Thanks luc. I adopted protocol from here. ...

Thanks luc.
I adopted protocol from here.
https://onlinelibrary.wiley.com/doi/full/10.1111/1755-0998.12529

However, it doesn't fit well with our lab's reagents so I changed some conditions....
Forum: General 12-20-2018, 08:50 PM
Replies: 3
Views: 1,425
Posted By Kujin Kwon
PCR after size selection of cDNA library

Hi all,

Recently I'm trying to make cDNA library using Tag-seq protocol.
After amplifying my sample with illumina adapter, I select 300~500bp size (0.5x TAE, Zymo Gel DNA recovery kit)
However,...
Forum: Introductions 12-20-2018, 08:03 PM
Replies: 0
Views: 1,695
Posted By Kujin Kwon
Hello

Hi all,
I'm graduate student studying about Parkinson's disease with sequencing data.
Recently, our lab bought iSeq machine, so I'm trying to make cDNA and genome library from various sample.
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