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Forum: RNA Sequencing 09-03-2012, 01:03 PM
Replies: 6
Views: 2,572
Posted By Deli Çoban
Hi, You can use the RNA-Seq libraries for...

Hi,

You can use the RNA-Seq libraries for identifiyig differentially expressed full pre-miRNA or partly degraded pre-miRNAs which generally include mature miRNAs. First of all, you blast the all...
Forum: Sample Prep / Library Generation 07-23-2011, 12:39 PM
Replies: 2
Views: 1,410
Posted By Deli Çoban
Hi, 1) Separate total RNA on a 15%...

Hi,

1) Separate total RNA on a 15% denaturing PAGE (7M Urea) for 90 minutes at 275V.

2) stain the gel with GelStar™ (it works pretty well but, I also tried by using Et. Bromide)

3) Select...
Forum: Bioinformatics 06-16-2011, 12:51 PM
Replies: 5
Views: 77,695
Posted By Deli Çoban
Really thanks for your valuable contribution.. ;)

Really thanks for your valuable contribution.. ;)
Forum: Sample Prep / Library Generation 06-04-2011, 04:47 PM
Replies: 4
Views: 5,673
Posted By Deli Çoban
Hi, ishmael During the library preparation,...

Hi, ishmael

During the library preparation, approximately 1 ug (< 50 pb) enriched small RNA sould be suffecient to get proper sequences for illumnia hiseq2000, according to friends who runs this...
Forum: Sample Prep / Library Generation 05-30-2011, 01:35 PM
Replies: 4
Views: 5,673
Posted By Deli Çoban
First, thanks ishmael for your response We...

First, thanks ishmael for your response

We want to sequence two endemic fish species whose genome are not sequenced and annotated in database. Our study mainly covers the microRNA sequencing and...
Forum: Sample Prep / Library Generation 05-23-2011, 08:52 AM
Replies: 4
Views: 5,673
Posted By Deli Çoban
Arrow initial amount of total RNA or enriched small rna for small rna seq

Hi everyone,

We are planing to small RNA sequencing using Illumina Genome Analyzer (Solexa), my question is how much the initial amount of total RNA should be in general ? or enriched small rna?
...
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