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Forum: Metagenomics 11-19-2019, 12:20 AM
Replies: 2
Views: 3,109
Posted By Light92
Question How to distinguish between 16S chloroplast reads and real ones?

Hello,

In an old experiment dating back to 2014, some 16S libraries from plant samples were prepared, with the aim to identify the microbial community living/contaminating the surface of the...
Forum: RNA Sequencing 09-13-2019, 01:38 AM
Replies: 0
Views: 2,328
Posted By Light92
Trinity RSEM Bowtie2 align_and_estimate_abundance script error

Hello!
I'm running a RNA-Seq differential gene expression pipeline analysis. I previously filtered my input sequences, then de-novo assembled my transcriptome through Trinity, and now, through a...
Forum: Bioinformatics 09-04-2019, 11:11 PM
Replies: 0
Views: 732
Posted By Light92
Molecular timetable method tools for circadian gene expression?

Hello, good morning to everyone! As I stated in my previous question (http://seqanswers.com/forums/showthread.php?p=228183), I'm working on a time-point experiment about circadian gene expression in...
Forum: Bioinformatics 09-03-2019, 07:35 AM
Replies: 3
Views: 899
Posted By Light92
Hmm...

You got a point there, but I'm working on plants, so maybe the speed gene expression changes isn't exactly the same. Bacteria are more adaptable, grow and replicate far quicker, and I can expect the...
Forum: Bioinformatics 09-03-2019, 02:03 AM
Replies: 3
Views: 899
Posted By Light92
Smile Most recent tools for circadian gene expression analysis (in plants)

Hello everyone,

I'm going to work on a project involving circadian gene expression analysis in the next months, and I'm looking for the best and most advanced/recent tools to perform this. I am...
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