Forum: Illumina/Solexa
03-13-2019, 06:11 AM
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Replies: 1
Views: 1,121
NovaSeq placement issue
Hi,
has anyone had issues with placement of NovaSeq in LEED buildings or other buildings with variable temp and humidity profiles?
Grateful for any input, trying to determine the right location...
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Forum: Academic/Non-Profit Jobs
01-10-2018, 08:23 AM
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Replies: 0
Views: 4,348
SRA1 OncoGenomics Miami
We have an opening for an SRA1 in a dynamic basic science genomics core serving Sylvester Comprehensive Cancer Center in Miami. Lots of nice toys, 10x Genomics, BD Genomics, Illumina, nanoString,...
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Forum: Academic/Non-Profit Jobs
11-20-2015, 07:25 AM
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Replies: 0
Views: 1,708
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Forum: Core Facilities
10-15-2015, 09:09 PM
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Replies: 6
Views: 5,421
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Forum: Core Facilities
10-14-2015, 08:53 AM
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Replies: 6
Views: 5,421
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Forum: Core Facilities
03-18-2014, 08:44 AM
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Replies: 2
Views: 4,349
Thanks. We wont be using it for LIMS, we are...
Thanks. We wont be using it for LIMS, we are interested in using it to track the progress of jobs, and auditing and managing billing.
We are a small core but we are about to embark on an expansion...
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Forum: Core Facilities
03-17-2014, 09:22 AM
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Replies: 2
Views: 4,349
iLab for genomics core
Hi,
Does anyone have any experience, comments or general opinion on the value or usability of iLab core facility management software?
cheers,
The_Roads
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Forum: Genomic Resequencing
09-20-2012, 05:59 AM
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Replies: 11
Views: 8,611
standard Sigma pcr cleanup columns and...
standard Sigma pcr cleanup columns and Clontech/Macherey-Nagel nucleospin columns have both worked fine for us to supply template to our core. i am pretty certain they do not do any further clean up...
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Forum: Bioinformatics
09-10-2012, 06:28 AM
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Replies: 6
Views: 3,780
yes i would agree with the above post --and...
yes i would agree with the above post --and modify my previous post. we do seem to see a lot of chimeric reads and i expect most of them are chimeras from library prep. we see very variable...
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Forum: General
08-17-2012, 09:18 AM
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Replies: 0
Views: 1,722
chimeric reads from TruSeq library prep
Hi,
We have a bunch of assemblies where we have identified chimeric reads, some of which we have verified as genuine recombination and some of which we suspect are artifact from PCR jumping during...
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Forum: Ion Torrent
07-12-2012, 08:09 AM
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Replies: 0
Views: 1,740
dealing with homopolymer/low complexity errors
Hi,
i am looking through our first pgm run sequencing long pcr fragments and assembling against a ref seq.
we have previously done the same thing on hiseqs with no issues.
for the pgm data...
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Forum: Bioinformatics
06-21-2012, 01:42 PM
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Replies: 2
Views: 1,804
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Forum: General
06-21-2012, 01:33 PM
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Replies: 14
Views: 4,954
i would say most talk of the error rates of ngs...
i would say most talk of the error rates of ngs grossly overemphasize the problem. yes there is a conflict rate of >>1% when you compare plain read sequences but as soon as you introduce any sort of...
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Forum: Bioinformatics
03-27-2012, 03:34 PM
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Replies: 6
Views: 3,780
i think illumina have done a pretty good job of...
i think illumina have done a pretty good job of reducing mix up of paired ends. i dont think we ever see any evidence of it. i would not expect any sequencing errors to be around 2-3% of reads.
we...
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Forum: Bioinformatics
03-27-2012, 07:30 AM
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Replies: 6
Views: 3,780
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Forum: RNA Sequencing
10-08-2011, 07:02 PM
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Replies: 9
Views: 2,839
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Forum: Illumina/Solexa
01-20-2011, 07:57 AM
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Replies: 2
Views: 4,006
reagrding this nextera purchase anyone heard...
reagrding this nextera purchase anyone heard anything about illuminas plans or targets for multiplexing? i heard the nextera purchase would allow them to increase capacity of capture/mulitplex...
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Forum: General
06-07-2010, 11:10 AM
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Replies: 7
Views: 3,541
Hi Avinash,
where are your sanger primers in...
Hi Avinash,
where are your sanger primers in relation to the exon and the indel? it may be that both results are true and that you are assembling psedogene sequence that is not amplifying with...
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Forum: Bioinformatics
03-29-2010, 12:15 PM
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Replies: 9
Views: 5,841
Hi PPARG,
Thanks for the reply. The internal...
Hi PPARG,
Thanks for the reply. The internal priming i mentioned was meant as internal priming during sequencing, it was something that was suggested by an illumina tech when we were discussing...
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Forum: Bioinformatics
03-27-2010, 05:04 PM
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Replies: 9
Views: 5,841
Hi,
If i understand your description we have...
Hi,
If i understand your description we have seen the same thing in paired end dna sequencing.
We see a small percentage of pairs where the two reads are either on top of each other or have...
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Forum: Bioinformatics
12-10-2009, 09:16 AM
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Replies: 65
Views: 35,535
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Forum: Bioinformatics
12-10-2009, 08:12 AM
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Replies: 65
Views: 35,535
Hi Johnny, I assume you are using CLCGWB. if so...
Hi Johnny, I assume you are using CLCGWB. if so the conflict table is not the place to look. you should run a snp detection. if you have an annotated ref seq then the table will present you with all...
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Forum: Bioinformatics
12-09-2009, 09:32 AM
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Replies: 2
Views: 4,133
IPAR recycling
Does anyone have any experience or pointers for recycling IPAR servers into assembly workstations/server?
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Forum: Bioinformatics
12-09-2009, 09:28 AM
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Replies: 7
Views: 3,454
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Forum: Bioinformatics
10-09-2009, 11:21 AM
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Replies: 14
Views: 11,062
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