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Forum: Illumina/Solexa 11-17-2011, 10:28 AM
Replies: 12
Views: 3,818
Posted By sgupta
Update

We have discontinued the use of SPRIworks for now. I think it is a issue with the bead purification/size selection process and the amount of sample in the 200 to 400 region after sonication....
Forum: Illumina/Solexa 07-21-2011, 08:11 AM
Replies: 12
Views: 3,818
Posted By sgupta
Hi Phillip, Thanks for your efforts. I will...

Hi Phillip,

Thanks for your efforts. I will speak with SPRIworks tech support again. In the past when we have spoken to them, they say that the size selection is not 100% accurate and 20% of...
Forum: Illumina/Solexa 07-21-2011, 05:13 AM
Replies: 12
Views: 3,818
Posted By sgupta
Hi Phillip, That is actually the problem. We...

Hi Phillip,

That is actually the problem. We expected the fragment size to be between 200 and 400 (based on size selection). But we see larger fragment size. So, SPRIworks seems to not eliminate...
Forum: Illumina/Solexa 07-20-2011, 11:59 AM
Replies: 12
Views: 3,818
Posted By sgupta
Hi pmiguel, Thanks for your reply. We are...

Hi pmiguel,

Thanks for your reply. We are seeing this phenotype predominately with whole cell extracts. Basically, the sample is split into 2 parts, one becomes the WCE, and the other goes through...
Forum: Illumina/Solexa 07-20-2011, 06:45 AM
Replies: 12
Views: 3,818
Posted By sgupta
lib prep using SPRIworks

Hi,

We have been recently experiencing that sometimes library preps done using SPRIworks show up with a larger fragment size than expected on the Bioanalyzer. And if we go ahead and sequence those...
Forum: Bioinformatics 11-07-2008, 02:57 PM
Replies: 7
Views: 6,796
Posted By sgupta
ABI Color Space to Bases

Hi,

I am trying to convert color space sequences generated by ABI SOLiD sequencer to actually bases using the following color space data "matrix":

AA=0
AC=1
AG=2
AT=3
CC=0
CA=1
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