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Forum: Illumina/Solexa 07-07-2015, 09:37 AM
Replies: 5
Views: 1,908
Posted By hcarrington
They are amplicon libraries; we've always done...

They are amplicon libraries; we've always done 10% PhiX, but maybe that should be increased. I suspected something may have happened with the instrument, I guess I better ask for a PM from Illumina....
Forum: Illumina/Solexa 07-07-2015, 07:27 AM
Replies: 5
Views: 1,908
Posted By hcarrington
Overclustered sequencing (low PF, low Q30) V2 2x250

Over the weekend I set up a V2 2x250 16S sequencing run on our Illumina MiSeq (I work in a Core lab). I came back on Monday and the results were terrible. Overall Q30 was 58.91, it overclustered at...
Forum: Core Facilities 12-10-2014, 07:39 AM
Replies: 5
Views: 4,557
Posted By hcarrington
I think the main connector is going to be a...

I think the main connector is going to be a barcode scanner to track lot numbers so I don't think it even needs to be that large. I will look at toughpads, thanks.
Forum: Core Facilities 12-10-2014, 07:20 AM
Replies: 5
Views: 4,557
Posted By hcarrington
Suggested Tablet for Core facility

I work in a Core DNA sequencing lab and wondered if anyone has incorporated use of a tablet into their day-to-day work? If so, what type of tablet seemed to work best, what kind of qualities were you...
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