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Forum: Pacific Biosciences 09-21-2011, 05:43 AM
Replies: 3
Views: 3,138
Posted By gallus
PacBio cuts 28% work force

Have they gone the way of Helicos?
Forum: 454 Pyrosequencing 09-02-2010, 05:58 AM
Replies: 21
Views: 11,728
Posted By gallus
we've been having problems with SV lots (93768220...

we've been having problems with SV lots (93768220 and 93776220). they look like the emPCR is the issue though no obvious broken reactors. the attached 1/8 Ti bacterial genomic was deemed "good" by...
Forum: 454 Pyrosequencing 08-11-2010, 05:02 AM
Replies: 2
Views: 2,237
Posted By gallus
the signal is the intensity of a given nucleotide...

the signal is the intensity of a given nucleotide on a bead. Typically key (CATG/ATGC and the like) signals are 400-500 and the library (GACT/TCAG) is above that by about 1.5-2X. the keySignalperBase...
Forum: 454 Pyrosequencing 08-03-2010, 10:35 AM
Replies: 2
Views: 2,237
Posted By gallus
library: control signal ratio

i've been poking around with the last 60 or so 454 runs that we've done at our core facility. i saw a pattern that i wanted to share and see if anyone else sees a similar pattern.
we've had pretty...
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