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Forum: Bioinformatics 09-21-2010, 05:03 AM
Replies: 1
Views: 2,683
Posted By pDNA
Software for comparative genomics of prokaryotes

Dear people,

can somebody suggest some software tool for comparative genomics of bacteria? It would be great to have a standalone software that is not web-based. I'm looking for a tool that is...
Forum: Sample Prep / Library Generation 05-10-2010, 09:06 AM
Replies: 0
Views: 2,072
Posted By pDNA
Isolation of genomic DNA

Dear people,

are there some agents one should avoid when isolating DNA for 454 sequencing (SDS, Triton, Phenol, Chloroform)? Is there some interference with certain substances?

Forum: 454 Pyrosequencing 04-29-2010, 10:10 AM
Replies: 2
Views: 1,770
Posted By pDNA
Sequencing of E. coli strains

I currently have a inquiry for the sequencing of 2 E. coli strains in hand. They want to use a 20kb paired end library for the sequencing task. I always thought that 3-8kb would be sufficient for the...
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