Forum: Sample Prep / Library Generation
04-21-2016, 06:04 AM
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Replies: 1
Views: 1,243
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Forum: Sample Prep / Library Generation
06-10-2014, 12:38 AM
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Replies: 6
Views: 3,406
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Forum: Sample Prep / Library Generation
06-21-2013, 05:57 AM
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Replies: 2
Views: 6,260
The HS chip is quite sensitive with regards to...
The HS chip is quite sensitive with regards to genomic DNA and overloading. So I would recommend not to load more than 50 ng/µl, better around 10-15 ng/µl if you want to quantify. There are several...
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Forum: 454 Pyrosequencing
10-01-2012, 06:30 AM
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Replies: 10
Views: 6,181
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Forum: Sample Prep / Library Generation
08-28-2012, 01:32 AM
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Replies: 49
Views: 74,277
Re. size of Ampure beads, I've also been...
Re. size of Ampure beads, I've also been wondering and reading a bit, and by looking through the Hawkins 1998 patent, I stumbled upon Biomag particles (formerly from PerSeptive, that had been...
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Forum: Sample Prep / Library Generation
05-07-2012, 06:18 AM
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Replies: 6
Views: 6,883
In case you are dealing with a smear, your...
In case you are dealing with a smear, your sensitivity of 0.5 ng/µl for the DNA1000 doesn't hold true, as these are defined to be in a (sharp) peak.
For a smear, the sensitivity will be lower...
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Forum: Sample Prep / Library Generation
03-09-2012, 12:32 AM
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Replies: 13
Views: 15,253
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Forum: Illumina/Solexa
01-25-2012, 05:00 AM
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Replies: 21
Views: 8,954
We tested ssDNA (primer size, <100 nt) on the...
We tested ssDNA (primer size, <100 nt) on the Bioanalyzer DNA assays, and it does run slower than dsDNA of the same size (roughly -25-30%).
Also, the quantification for the ssDNA is off (may be...
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Forum: Sample Prep / Library Generation
01-02-2012, 07:06 AM
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Replies: 8
Views: 7,702
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Forum: Sample Prep / Library Generation
01-02-2012, 06:20 AM
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Replies: 8
Views: 7,702
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Forum: Sample Prep / Library Generation
10-31-2011, 05:44 AM
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Replies: 10
Views: 11,316
don't understand
Hi pmiguel, I don't exactly understand why you would run dsDNA on a RNA chip? It's not made for running DNA and prone to get the wrong results.
For what reason do you want to do that, what's the...
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Forum: Sample Prep / Library Generation
02-07-2011, 08:07 AM
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Replies: 49
Views: 74,277
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Forum: Sample Prep / Library Generation
08-27-2010, 05:06 AM
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Replies: 13
Views: 15,253
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Forum: Sample Prep / Library Generation
08-27-2010, 04:57 AM
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Replies: 13
Views: 15,253
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Forum: 454 Pyrosequencing
11-10-2009, 05:50 AM
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Replies: 28
Views: 11,619
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Forum: General
11-06-2009, 07:52 AM
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Replies: 8
Views: 8,041
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Forum: General
11-06-2009, 02:01 AM
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Replies: 8
Views: 8,041
Success of Solexa-platform
Hi members,
I am currently preparing an overview presentation for my science colleagues (unfamiliar with NGS) on the next- and now-gen sequencing technologies. Obviously, this forum is a great place...
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Forum: General
11-05-2009, 07:46 AM
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Replies: 9
Views: 25,112
GATC webpage
I also think to have ssen more on comparisons somewhere on seqanswers, but also GATC offers info on their page:
http://www.gatc-biotech.com/en/sequencing.html
At least it mentions in part for what...
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Forum: 454 Pyrosequencing
10-29-2009, 03:37 AM
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Replies: 28
Views: 11,619
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Forum: Site Feedback/Suggestions
10-19-2009, 07:16 AM
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Replies: 14
Views: 4,966
Thanks a lot!!
It seems like it's coming down...
Thanks a lot!!
It seems like it's coming down to personal preferences finally - one person would like to see the forums, somebody else may prefer having the post (snippet? - I think the feed...
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Forum: Site Feedback/Suggestions
10-16-2009, 07:45 AM
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Replies: 14
Views: 4,966
report
Attensa didn't get 'all' new posts (Oct. 14+15+16) at once, however, after a second round of spitting them out, I received 50 posts in the feed (suspicious number, hmmm? :confused:).
It's probably...
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Forum: Site Feedback/Suggestions
10-16-2009, 04:49 AM
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Replies: 14
Views: 4,966
Works!
Attensa was chewing on it a moment and then... there it was: all new posts! Cool :-)
THANKS!
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Forum: Site Feedback/Suggestions
10-16-2009, 02:56 AM
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Replies: 2
Views: 3,472
New topic suggestion
How about a separate topic for target enrichment? It seems to be sneaking between the supplier's forums (Nimblegen) and sample prep and I feel it's being mentioned more lately.
I admit that it's a...
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Forum: Site Feedback/Suggestions
10-16-2009, 02:45 AM
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Replies: 14
Views: 4,966
Any news on the "feed" story?
Hi ECO,
sure you're busy, also with the spam issue coming up (:mad: for what reason anybody spams this forum I will never understand :mad:)...
But I wanted to check back on the RSS-question asked...
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Forum: Introductions
10-16-2009, 01:55 AM
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Replies: 1
Views: 1,433
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