Forum: Ion Torrent
12-12-2013, 09:29 AM
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Replies: 6
Views: 6,612
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Forum: Ion Torrent
12-09-2013, 10:13 AM
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Replies: 5
Views: 3,780
Hi there,
After logging in to the AmpliSeq...
Hi there,
After logging in to the AmpliSeq Designer website (https://ampliseq.com/), navigate to the Ion Panels page. From there you can choose to 'Download panel files" for the panel you need....
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Forum: Ion Torrent
11-08-2013, 02:17 PM
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Replies: 7
Views: 5,383
Sorry, we might have missed the earlier post....
Sorry, we might have missed the earlier post. Please do consider Ion Community for those kinds of requests as well - there are team members monitoring the various spaces who are dedicated to...
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Forum: Ion Torrent
11-08-2013, 09:00 AM
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Replies: 7
Views: 5,383
Hi there GenoMax,
In fact, Torrent Suite...
Hi there GenoMax,
In fact, Torrent Suite software (and a few other packages) is open source and is freely available via our github repository:
https://github.com/iontorrent
As mentioned on the...
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Forum: Ion Torrent
10-08-2013, 11:36 AM
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Replies: 2
Views: 2,942
Hi there,
At the present we have the 16...
Hi there,
At the present we have the 16 barcode kit available for RNA. Please send me a private message and I would be happy to share information of how we might be able to assist with your need.
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Forum: Ion Torrent
09-13-2013, 07:26 AM
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Replies: 3
Views: 5,668
Hi there,
There is functionality within the...
Hi there,
There is functionality within the Torrent Suite software for duplicate read marking, which can be enabled as desired. There is more information available via the Help tool within Torrent...
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Forum: Ion Torrent
09-09-2013, 01:57 PM
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Replies: 1
Views: 5,888
Hi there,
"Switch" Solution is simply meant...
Hi there,
"Switch" Solution is simply meant to convey the change in conditions between the PCR and ligation steps of the library construction workflow.
For what it's worth, SEQanswers is an...
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Forum: Ion Torrent
08-29-2013, 10:21 AM
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Replies: 2
Views: 5,137
Hi there,
In our experience, off-target...
Hi there,
In our experience, off-target reads are typically a combination of specific per panel and per sample effects. For example, the best HMW DNAs will tend to have very similar off-target...
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Forum: Ion Torrent
08-22-2013, 09:31 AM
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Replies: 17
Views: 12,064
Hi there,
If you are an Ion Torrent...
Hi there,
If you are an Ion Torrent customer, the documentation for Torrent Suite may be found linked within Torrent Browser. For others, or for offline access, the relevant documents are also...
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Forum: Ion Torrent
08-22-2013, 09:00 AM
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Replies: 6
Views: 7,453
Hi there,
Ion Community is completely open...
Hi there,
Ion Community is completely open to all registrants - i.e. there are no member access levels. You should have full access to all technical information contained within.
With the...
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Forum: Ion Torrent
07-23-2013, 01:16 PM
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Replies: 6
Views: 7,453
Hi there,
There are, in fact, two library...
Hi there,
There are, in fact, two library adaptors - we call them A and P1. In most library preparations these are ligated onto library insert fragments. In some cases, these may be amplified onto...
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Forum: Ion Torrent
06-14-2013, 08:26 AM
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Replies: 122
Views: 113,028
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Forum: Ion Torrent
05-09-2013, 07:26 PM
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Replies: 3
Views: 3,351
Hi Brandon,
While there's not an official...
Hi Brandon,
While there's not an official supported protocol to do that with MuSeek, you're absolutely correct - full length ds cDNA can generally be treated like gDNA for most library prep...
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Forum: Ion Torrent
03-01-2013, 04:38 PM
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Replies: 27
Views: 19,250
Hi Alessandro,
TMAP is open source:...
Hi Alessandro,
TMAP is open source: https://github.com/iontorrent/TMAP
Also, if you were unable to access the link posted by jdilts, you can register for an account at the Ion Community...
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Forum: Ion Torrent
01-22-2013, 07:28 AM
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Replies: 9
Views: 3,735
Hi Véronique,
What method of poly(A)...
Hi Véronique,
What method of poly(A) enrichment did you use? Is this a human RNA sample? What you describe generally has its roots in the initial RNA enrichment/depletion, as opposed to the...
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Forum: Metagenomics
01-11-2013, 10:35 AM
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Replies: 3
Views: 2,569
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Forum: Ion Torrent
12-07-2012, 07:49 AM
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Replies: 6
Views: 5,652
Hi Joltex,
Regarding the adaptor...
Hi Joltex,
Regarding the adaptor concentration in the kit- for the kits supported by the current Ion Xpress Plus Fragment Library Preparation User Guide...
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Forum: Ion Torrent
12-07-2012, 07:20 AM
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Replies: 2
Views: 4,524
Hi Apex,
Libraries for the Ion Proton...
Hi Apex,
Libraries for the Ion Proton Sequencer are indeed made using the same adaptors presently used on Ion PGM Sequencer. This means that you can sequence PGM libraries via the OneTouch 2 and...
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Forum: Ion Torrent
11-16-2012, 09:28 AM
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Replies: 3
Views: 2,468
Hi soleulloa,
I've attached an image of the...
Hi soleulloa,
I've attached an image of the current library table. which highlights the target insert size for each sequencing read length. Parameters for the 400 base read methods will be added...
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Forum: Ion Torrent
09-27-2012, 01:00 PM
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Replies: 17
Views: 11,327
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Forum: Ion Torrent
08-27-2012, 08:36 AM
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Replies: 1
Views: 2,082
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Forum: Ion Torrent
08-20-2012, 07:56 AM
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Replies: 8
Views: 8,198
Hi there,
Indeed, this does seem unusual and...
Hi there,
Indeed, this does seem unusual and I'd like to help you with your question. I tried to send you a follow up note but your profile settings do not seem to allow receipt of messages from...
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Forum: Ion Torrent
08-08-2012, 08:17 AM
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Replies: 4
Views: 9,276
Hi Apex,
The sequences of the fragment...
Hi Apex,
The sequences of the fragment library adaptors can be found in Appendix D (pp. 45) of the current Ion Xpress™ Plus gDNA Fragment Library Preparation User Guide...
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Forum: Ion Torrent
07-18-2012, 04:14 AM
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Replies: 10
Views: 12,174
Hi slm1816,
Thanks for reporting the issue...
Hi slm1816,
Thanks for reporting the issue with the KRAS G12D exon number in Ion Reporter™ Software. We confirmed this error recently and have identified a solution. You can expect an update...
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Forum: Ion Torrent
07-05-2012, 03:26 PM
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Replies: 27
Views: 19,250
Hi hengnck,
Are you using all 480 Mbp of...
Hi hengnck,
Are you using all 480 Mbp of data in the assembly or are you downsampling? I ask because many software packages (like those you mention) will grossly underperform with excessive...
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