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Forum: Sample Prep / Library Generation 03-08-2012, 01:11 AM
Replies: 4
Views: 4,717
Posted By andyding
What about whole genome amplification before...

What about whole genome amplification before library prep?
Cheers.
Forum: Illumina/Solexa 03-07-2012, 06:29 PM
Replies: 3
Views: 2,875
Posted By andyding
Thanks for your reply. I was wondering if you...

Thanks for your reply.
I was wondering if you can tell me what is the potential technical difficulties when constructing libraries with ultra-large insert size?
Thanks again.

Andy
Forum: Bioinformatics 03-06-2012, 09:59 PM
Replies: 2
Views: 1,692
Posted By andyding
You don't need to do reverse complement for the...

You don't need to do reverse complement for the second set of read.
As Jon_Keats said, most mainstream aligners are designed to deal with Illumina paired-end reads.
I never used tophat, but I've...
Forum: Illumina/Solexa 03-06-2012, 02:06 AM
Replies: 3
Views: 2,875
Posted By andyding
Question Maximum Insert Size of Illumina Libraries?

Hi, all:
I'm wondering what is the maximum insert size of illumina paired-end or mated-paired libraries? Some source saids it's 20kb or 40kb, I'm wondering if it is possible that I can get a even...
Forum: Introductions 11-24-2011, 12:30 AM
Replies: 3
Views: 1,065
Posted By andyding
Nice to meet u too!

Nice to meet u too!
Forum: Bioinformatics 11-21-2011, 10:35 AM
Replies: 0
Views: 2,568
Posted By andyding
Question Problem Encountered When Using SOAPdenovo: Floating Point / Segmentation Error

Hi, Dear Colleagues:
I'm a student from Fudan University, and is currently trying to use SOAPdenovo to run de novo assemble on human mitochondrial genome sequencing data. These data are either...
Forum: Introductions 11-21-2011, 10:29 AM
Replies: 3
Views: 1,065
Posted By andyding
Which institution are u working at? I'm at Fudan...

Which institution are u working at? I'm at Fudan University at Shanghai!
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