SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 7 of 7
Search took 0.00 seconds.
Search: Posts Made By: teuthbrush
Forum: Sample Prep / Library Generation 08-19-2019, 11:05 AM
Replies: 2
Views: 417
Posted By teuthbrush
For what it's worth, I just use a pipette, and...

For what it's worth, I just use a pipette, and even with a drying time of less than one minute, I haven't had any inhibition of my PCRs using Phusion as an enzyme, at least. Occasionally I'll see...
Forum: Sample Prep / Library Generation 07-15-2019, 12:17 PM
Replies: 6
Views: 636
Posted By teuthbrush
https://ethanomics.files.wordpress.com/2012/08/ser...

https://ethanomics.files.wordpress.com/2012/08/serapure_v2-2.pdf

OpenWetware also has one that is a bit more in-depth: https://openwetware.org/wiki/SPRI_bead_mix
Forum: Sample Prep / Library Generation 07-15-2019, 10:14 AM
Replies: 6
Views: 636
Posted By teuthbrush
Have you looked into making your own beads,...

Have you looked into making your own beads, starting with Seramag Speedbeads? It's very cheap and works pretty well.
Forum: Sample Prep / Library Generation 05-22-2019, 04:49 PM
Replies: 4
Views: 1,015
Posted By teuthbrush
So, thought I'd update you all. I tried SPRI...

So, thought I'd update you all.

I tried SPRI again with Agencourt beads, got a slightly better result. But here's the kicker:

As I was trying different things, on one plate, I accidentally...
Forum: Sample Prep / Library Generation 04-30-2019, 08:43 AM
Replies: 4
Views: 1,015
Posted By teuthbrush
I did include the CutSmart buffer, yeah. As far...

I did include the CutSmart buffer, yeah. As far as I know, both the CutSmart and the enzyme itself contain BSA, or at least that was the case last I checked. The thread you linked is this one, by the...
Forum: Sample Prep / Library Generation 04-29-2019, 03:40 PM
Replies: 4
Views: 1,015
Posted By teuthbrush
SPRI Troubleshooting

Hey folks, I'm running into a weird issue with my SPRI cleanups and I was wondering if anyone had any advice.

So, I'm cleaning up a double digest (BSSSIa, MseI) of 100ng of DNA. The DNA itself is...
Forum: Sample Prep / Library Generation 11-07-2018, 10:56 AM
Replies: 2
Views: 981
Posted By teuthbrush
It is much, much cheaper to make your own SPRI...

It is much, much cheaper to make your own SPRI beads, and they work just as well.

https://openwetware.org/wiki/SPRI_bead_mix
Showing results 1 to 7 of 7

 


All times are GMT -8. The time now is 10:09 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO