SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 3 of 3
Search took 0.00 seconds.
Search: Posts Made By: nucacidhunter
Forum: Illumina/Solexa 09-03-2017, 02:14 PM
Replies: 9
Views: 2,157
Posted By nucacidhunter
For dark cycles you would need Illumina's...

For dark cycles you would need Illumina's assistance in your own risk if they agree and generally is worthwhile if you are doing multiple runs. I have not heard of dark cycles on NextSeq.
Forum: Illumina/Solexa 09-03-2017, 01:44 PM
Replies: 9
Views: 2,157
Posted By nucacidhunter
Two issues with RRBS sequencing: 1- unbalanced...

Two issues with RRBS sequencing:
1- unbalanced base composition: NextSeq performs fine
2- low diversity at the start of reads: depends on method and if you are using NuGEN kit 1% PhiX would fine...
Forum: Illumina/Solexa 03-29-2017, 11:47 PM
Replies: 9
Views: 2,157
Posted By nucacidhunter
NextSeq seems to perform good with low diversity...

NextSeq seems to perform good with low diversity and unbalanced libraries such as RRBS as shown in the following:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5065634/
Showing results 1 to 3 of 3

 


All times are GMT -8. The time now is 08:42 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO